Abstract
Currents elicited by L-glutamate and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (~0 mV) and apparent elementary conductance (2-3 pS). The power-density spectra of the noise increases produced by each agonist were fit by the sum of two Lorentzian curves having similar cutoff frequencies (τ1 ~ 5 msec, τ2 ~ 1 msec), but the relative power of these components were different for quisqualate and glutamate than for kainate. Moreover, the responses to high doses of either quisqualate or glutamate rapidly faded, whereas the responses to kainate did not. Finally, quisqualate and glutamate produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate, and in our preparation, glutamate appear to activate channels different than those activated by N-methyl-D-aspartate in other preparations. At least some of the effects of quisqualate and glutamate appear to be mediated by receptors bound by kainate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1837-1841 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 82 |
| Issue number | 6 |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Quisqualate and L-glutamate inhibit retinal horizontal-cell responses to kainate. / Ishida, Andrew; Neyton, J.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 6, 1985, p. 1837-1841.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quisqualate and L-glutamate inhibit retinal horizontal-cell responses to kainate
AU - Ishida, Andrew
AU - Neyton, J.
PY - 1985
Y1 - 1985
N2 - Currents elicited by L-glutamate and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (~0 mV) and apparent elementary conductance (2-3 pS). The power-density spectra of the noise increases produced by each agonist were fit by the sum of two Lorentzian curves having similar cutoff frequencies (τ1 ~ 5 msec, τ2 ~ 1 msec), but the relative power of these components were different for quisqualate and glutamate than for kainate. Moreover, the responses to high doses of either quisqualate or glutamate rapidly faded, whereas the responses to kainate did not. Finally, quisqualate and glutamate produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate, and in our preparation, glutamate appear to activate channels different than those activated by N-methyl-D-aspartate in other preparations. At least some of the effects of quisqualate and glutamate appear to be mediated by receptors bound by kainate.
AB - Currents elicited by L-glutamate and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (~0 mV) and apparent elementary conductance (2-3 pS). The power-density spectra of the noise increases produced by each agonist were fit by the sum of two Lorentzian curves having similar cutoff frequencies (τ1 ~ 5 msec, τ2 ~ 1 msec), but the relative power of these components were different for quisqualate and glutamate than for kainate. Moreover, the responses to high doses of either quisqualate or glutamate rapidly faded, whereas the responses to kainate did not. Finally, quisqualate and glutamate produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate, and in our preparation, glutamate appear to activate channels different than those activated by N-methyl-D-aspartate in other preparations. At least some of the effects of quisqualate and glutamate appear to be mediated by receptors bound by kainate.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021858749&partnerID=8YFLogxK
M3 - Article
VL - 82
SP - 1837
EP - 1841
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 6
ER -