Quisqualate and L-glutamate inhibit retinal horizontal-cell responses to kainate

Andrew Ishida, J. Neyton

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Currents elicited by L-glutamate and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (~0 mV) and apparent elementary conductance (2-3 pS). The power-density spectra of the noise increases produced by each agonist were fit by the sum of two Lorentzian curves having similar cutoff frequencies (τ1 ~ 5 msec, τ2 ~ 1 msec), but the relative power of these components were different for quisqualate and glutamate than for kainate. Moreover, the responses to high doses of either quisqualate or glutamate rapidly faded, whereas the responses to kainate did not. Finally, quisqualate and glutamate produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate, and in our preparation, glutamate appear to activate channels different than those activated by N-methyl-D-aspartate in other preparations. At least some of the effects of quisqualate and glutamate appear to be mediated by receptors bound by kainate.

Original languageEnglish (US)
Pages (from-to)1837-1841
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number6
StatePublished - 1985
Externally publishedYes

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Retinal Horizontal Cells
Quisqualic Acid
Kainic Acid
Glutamic Acid
Excitatory Amino Acid Agonists
Kainic Acid Receptors
Goldfish
Patch-Clamp Techniques
N-Methylaspartate
Noise

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Quisqualate and L-glutamate inhibit retinal horizontal-cell responses to kainate. / Ishida, Andrew; Neyton, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 6, 1985, p. 1837-1841.

Research output: Contribution to journalArticle

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AB - Currents elicited by L-glutamate and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (~0 mV) and apparent elementary conductance (2-3 pS). The power-density spectra of the noise increases produced by each agonist were fit by the sum of two Lorentzian curves having similar cutoff frequencies (τ1 ~ 5 msec, τ2 ~ 1 msec), but the relative power of these components were different for quisqualate and glutamate than for kainate. Moreover, the responses to high doses of either quisqualate or glutamate rapidly faded, whereas the responses to kainate did not. Finally, quisqualate and glutamate produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate, and in our preparation, glutamate appear to activate channels different than those activated by N-methyl-D-aspartate in other preparations. At least some of the effects of quisqualate and glutamate appear to be mediated by receptors bound by kainate.

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