Quantum dots as reporters in multiplexed immunoassays for biomarkers of exposure to agrochemicals

M. Nichkova, D. Dosev, A. E. Davies, S. J. Gee, I. M. Kennedy, B. D. Hammock

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3-phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using QDs (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.

Original languageEnglish (US)
Pages (from-to)1423-1433
Number of pages11
JournalAnalytical Letters
Volume40
Issue number7
DOIs
StatePublished - Jan 2007

Keywords

  • Atrazine
  • Immunoassay
  • Microarrays
  • Microconatct printing
  • Pyrethroid
  • Quantum dot

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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    Nichkova, M., Dosev, D., Davies, A. E., Gee, S. J., Kennedy, I. M., & Hammock, B. D. (2007). Quantum dots as reporters in multiplexed immunoassays for biomarkers of exposure to agrochemicals. Analytical Letters, 40(7), 1423-1433. https://doi.org/10.1080/00032710701327088