Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells

Edward B. Garon, Laura Marcu, Quang Luong, Oxana Tcherniantchouk, Gay M. Crooks, H. Phillip Koeffler

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Quantum dots (QDs) are nanometer scale fluorescent semiconductors that are increasingly used as labeling tools in biological research. These nanoparticles have physical properties, such as high quantum yield and resistance to photobleaching, that make them attractive molecular probes for tracking hematologic cells. Here, we show that QDs attached to a transporter protein effectively label all hematologic cells tested, including cell lines and malignant and non-malignant patient samples. We demonstrate that dividing cells can be tracked through at least four cell divisions. In leukemic cell lines, some cells remain labeled for 2 weeks. We show that QDs can be used to follow cells as they differentiate. QDs are seen in monocyte-like and neutrophil-like progeny of labeled HL-60 myeloblasts exposed to Vitamin D analogues and DMSO, respectively. QDs are also observed in monocytes generated from labeled CD34+ cells. In addition, QDs attached to streptavidin can target cells with differing cell surface markers, including CD33. In summary, QDs have the ability to bind to specific cells of interest, be taken up by a diverse range of hematologic cells, and followed through many divisions and through differentiation. These results establish QDs as extremely useful molecular imaging tools for the study of hematologic cells.

Original languageEnglish (US)
Pages (from-to)643-651
Number of pages9
JournalLeukemia Research
Volume31
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Quantum Dots
Fetal Blood
Blood Cells
Bone Marrow
Monocytes
Cell Tracking
Photobleaching
Molecular Probes
Cell Line
Granulocyte Precursor Cells
Semiconductors
Molecular Imaging
Streptavidin
Dimethyl Sulfoxide
Vitamin D
Cell Division
Nanoparticles
Neutrophils

Keywords

  • Cell tracking
  • Fluorescence microscopy
  • Hematopoiesis
  • Nanotechnology

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Garon, E. B., Marcu, L., Luong, Q., Tcherniantchouk, O., Crooks, G. M., & Koeffler, H. P. (2007). Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. Leukemia Research, 31(5), 643-651. https://doi.org/10.1016/j.leukres.2006.08.006

Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. / Garon, Edward B.; Marcu, Laura; Luong, Quang; Tcherniantchouk, Oxana; Crooks, Gay M.; Koeffler, H. Phillip.

In: Leukemia Research, Vol. 31, No. 5, 05.2007, p. 643-651.

Research output: Contribution to journalArticle

Garon, EB, Marcu, L, Luong, Q, Tcherniantchouk, O, Crooks, GM & Koeffler, HP 2007, 'Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells', Leukemia Research, vol. 31, no. 5, pp. 643-651. https://doi.org/10.1016/j.leukres.2006.08.006
Garon EB, Marcu L, Luong Q, Tcherniantchouk O, Crooks GM, Koeffler HP. Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. Leukemia Research. 2007 May;31(5):643-651. https://doi.org/10.1016/j.leukres.2006.08.006
Garon, Edward B. ; Marcu, Laura ; Luong, Quang ; Tcherniantchouk, Oxana ; Crooks, Gay M. ; Koeffler, H. Phillip. / Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. In: Leukemia Research. 2007 ; Vol. 31, No. 5. pp. 643-651.
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