Quantum dot-DNA bioconjugates for fluorescence resonance energy transfer-based biosensing

Igor L. Medintz, Lorenzo Berti, Thomas Pons, Hedi Mattoussi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Semiconductor quantum dots (QDs) have unique photophysical properties which make them excellent fluorescence resonance energy transfer donors. However, lack of facile methods for conjugating biomolecules such as DNA, proteins and peptides to QDs have limited their applications. In this report, we describe a general procedure for the preparation of a synthetic peptide that can be covalently attached to DNA segments and used to facilitate the self-assembly of the modified DNA onto water soluble QDs. To characterize this conjugation strategy, dye-labeled DNA is first reacted with the synthetic peptide and the resulting peptideDNA then self-assembled onto QDs. QD attachment is verified by monitoring resonance energy transfer efficiency from the QD donor to the dye-labeled DNA acceptor. QD-DNA bioconjugates assembled using this method may find applications as molecular beacons and hybridization probes.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6448
DOIs
Publication statusPublished - 2007
Externally publishedYes
EventColloidal Quantum Dots for Biomedical Applications II - San Jose, CA, United States
Duration: Jan 20 2007Jan 23 2007

Other

OtherColloidal Quantum Dots for Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/20/071/23/07

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Keywords

  • Biosensor
  • DNA
  • Molecular beacon
  • Nanocrystal
  • Quantum dot
  • Resonance energy transfer
  • Semiconductor

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Medintz, I. L., Berti, L., Pons, T., & Mattoussi, H. (2007). Quantum dot-DNA bioconjugates for fluorescence resonance energy transfer-based biosensing. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6448). [64480G] https://doi.org/10.1117/12.699384