Quantitative real time measurements of bacteria-bacteriophages interactions in fresh lettuce leaves

Zhen Luo, Christopher W. Simmons, Jean S. Vandergheynst, Nitin Nitin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacteriophages are promising bactericidal agents for controlling growth of pathogenic and spoilage bacteria in fresh produce and in minimally-processed food. However, there is a lack of quantitative understanding of phage-bacteria interactions within a food matrix. To address this unmet need, the interactions of lytic bacteriophages with target model bacteria on the surface and interior (infiltrated) of a fresh produce were characterized using bioluminescence spectroscopy and imaging approaches. The results showed that the phages applied to the surface of lettuce leaves effectively reduced the level of bacterial targets that were present both on the surface and inside of lettuce leaves. However, there were significant differences in the initial rate of decrease of bacteria in the three model systems examined in this study: (a) homogenous well-mixed solution, (b) inoculated on the surface, and (c) infiltrated into the interior of lettuce leaves. The results also demonstrate the application of bioluminescence imaging for non-invasive measurement of interactions between bactericidal agents and target pathogens over an extended period of time in lettuce samples. The number of bacterial cells in lettuce samples before and after phage treatment predicted based on bioluminescence measurements was in agreement with the number calculated by conventional plate counting methods. In summary, the results highlight a non-invasive real-time imaging approach to quantitatively measure interactions of bacteriophages with both the surface inoculated and the infiltrated model bacterial cells in leafy greens.

Original languageEnglish (US)
Pages (from-to)176-185
Number of pages10
JournalJournal of Food Engineering
Volume111
Issue number1
DOIs
StatePublished - Jul 2012

Fingerprint

leaf lettuce
Lettuce
bacteriophages
Bacteriophages
Bacteria
bioluminescence
bacteria
fresh produce
image analysis
lettuce
minimally processed foods
Luminescent Measurements
Food
food matrix
green leafy vegetables
spectroscopy
Spectrum Analysis
Cell Count
cells
sampling

Keywords

  • Bacteria
  • Bacteriophages
  • Bioluminescence imaging and Spectroscopy
  • Fresh produce
  • Internalization

ASJC Scopus subject areas

  • Food Science

Cite this

Quantitative real time measurements of bacteria-bacteriophages interactions in fresh lettuce leaves. / Luo, Zhen; Simmons, Christopher W.; Vandergheynst, Jean S.; Nitin, Nitin.

In: Journal of Food Engineering, Vol. 111, No. 1, 07.2012, p. 176-185.

Research output: Contribution to journalArticle

Luo, Zhen ; Simmons, Christopher W. ; Vandergheynst, Jean S. ; Nitin, Nitin. / Quantitative real time measurements of bacteria-bacteriophages interactions in fresh lettuce leaves. In: Journal of Food Engineering. 2012 ; Vol. 111, No. 1. pp. 176-185.
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