Quantitative profiling method for oxylipin metabolome by liquid chromatography electrospray ionization tandem mass spectrometry

Jun Yang, Kara Schmelzer, Katrin Georgi, Bruce D. Hammock

Research output: Contribution to journalArticle

214 Scopus citations

Abstract

Cyclooxygenase, lipoxygenase, and epoxygenase derived oxylipins, especially eicosanoids, play important roles in many physiological processes. Assessment of oxidized fatty acid levels is important for understanding their homeostatic and pathophysiological roles. Most reported methods examine these pathways in isolation. The work described here employed a solid phase extraction-liquid chromatography-electrospray ionization MS/MS (SPE-LC-ESI MS/MS) method to monitor these metabolites. In 21 min, 39 oxylipins were quantified along with eight corresponding internal standards. The limits of quantification were between 0.07 and 32 pg (20 pM-10 nM). Finally, the validated method was used to evaluate oxylipin profiles in lipopolysaccharide-exposed mice, an established septic inflammatory model. The method described here offers a useful tool for the evaluation of complex regulatory oxylipin responses in in vitro or in vivo studies.

Original languageEnglish (US)
Pages (from-to)8085-8093
Number of pages9
JournalAnalytical Chemistry
Volume81
Issue number19
DOIs
StatePublished - Oct 1 2009

ASJC Scopus subject areas

  • Analytical Chemistry

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