Quantitative panoramic imaging of epicardial electrical activity

Qing Lou, Crystal M Ripplinger, Philip V. Bayly, Igor R. Efimov

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Fluorescent imaging with voltage- and/or calcium-sensitive dyes has revolutionized cardiac physiology research. Here we present improved panoramic imaging for optically mapping electrical activity from the entire epicardium of the Langendorff-perfused rabbit heart. Combined with reconstruction of the 3D heart surface, the functional data can be conveniently visualized on the realistic heart geometry. Methods to quantify the panoramic data set are introduced by first describing a simple approach to mesh the heart in regular grid form. The regular grid mesh provides substrate for easy translation of previously available non-linear dynamics methods for 2D array data. It also simplifies the unwrapping of curved three-dimensional surface to 2D surface for global epicardial visualization of the functional data. The translated quantification methods include activation maps (isochrones), phase maps, phase singularity, and electric stimulus-induced virtual electrode polarization (VEP) maps. We also adapt a method to calculate the conduction velocities on the global epicardial surface by taking the curvature of the heart surface into account.

Original languageEnglish (US)
Pages (from-to)1649-1658
Number of pages10
JournalAnnals of Biomedical Engineering
Volume36
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

Keywords

  • Action potential
  • Conduction velocity
  • Mesh
  • Optical mapping
  • Panoramic fluorescent imaging
  • Unwrapping

ASJC Scopus subject areas

  • Biomedical Engineering

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