Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography

Shau Poh Chong, Conrad W. Merkle, Conor Leahy, Harsha Radhakrishnan, Vivek Srinivasan

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.

Original languageEnglish (US)
Article numberA030
Pages (from-to)1429-1450
Number of pages22
JournalBiomedical Optics Express
Volume6
Issue number4
DOIs
StatePublished - Jan 1 2015

Fingerprint

Optical Coherence Tomography
hemoglobin
Hemoglobins
tomography
Light
chromophores
Oxyhemoglobins
scattering
hematocrit
oxyhemoglobin
Hematocrit
rodents
specular reflection
Noise
Retina
retina
Rodentia
Angiography
Oxygen
quantitative analysis

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography. / Chong, Shau Poh; Merkle, Conrad W.; Leahy, Conor; Radhakrishnan, Harsha; Srinivasan, Vivek.

In: Biomedical Optics Express, Vol. 6, No. 4, A030, 01.01.2015, p. 1429-1450.

Research output: Contribution to journalArticle

Chong, Shau Poh ; Merkle, Conrad W. ; Leahy, Conor ; Radhakrishnan, Harsha ; Srinivasan, Vivek. / Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 4. pp. 1429-1450.
@article{28e376074d7c4e269d7d6a727faff8c0,
title = "Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography",
abstract = "Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.",
author = "Chong, {Shau Poh} and Merkle, {Conrad W.} and Conor Leahy and Harsha Radhakrishnan and Vivek Srinivasan",
year = "2015",
month = "1",
day = "1",
doi = "10.1364/BOE.6.001429",
language = "English (US)",
volume = "6",
pages = "1429--1450",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "4",

}

TY - JOUR

T1 - Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography

AU - Chong, Shau Poh

AU - Merkle, Conrad W.

AU - Leahy, Conor

AU - Radhakrishnan, Harsha

AU - Srinivasan, Vivek

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.

AB - Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.

UR - http://www.scopus.com/inward/record.url?scp=84941314779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941314779&partnerID=8YFLogxK

U2 - 10.1364/BOE.6.001429

DO - 10.1364/BOE.6.001429

M3 - Article

AN - SCOPUS:84941314779

VL - 6

SP - 1429

EP - 1450

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 4

M1 - A030

ER -