Quantitative microvascular hemoglobin mapping using visible light spectroscopic optical coherence tomography

Shau Poh Chong, Conrad W. Merkle, Conor Leahy, Harsha Radhakrishnan, Vivek Srinivasan

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.

Original languageEnglish (US)
Article numberA030
Pages (from-to)1429-1450
Number of pages22
JournalBiomedical Optics Express
Volume6
Issue number4
DOIs
StatePublished - Jan 1 2015

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ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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