Abstract
Biological radioisotope studies suffer from a lack of sensitive measurement techniques and therefore traditionally require large amounts of labeled material to produce a measurable signal. Such quantities of materials are often significantly higher than naturally-occurring levels preventing these studies from replicating physiological conditions. AMS affords the sensitivity necessary to perform biological radioisotope studies with low levels of labeled material that preserve physiological conditions. The choice of labeled material can substantially affect the ease of interpretation and comprehensiveness of these studies. Here, the benefits and limitations of whole-cell labeling with 14C-glucose and targeted pathway labeling with 14C-nicotinic acid are discussed and compared.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1309-1312 |
| Number of pages | 4 |
| Journal | Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms |
| Volume | 268 |
| Issue number | 7-8 |
| DOIs | |
| State | Published - Apr 2010 |
| Externally published | Yes |
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Keywords
- AMS
- Biology
- Cell labeling
- Glucose
- HPLC
- Metabolism
- NAD
- Yeast
ASJC Scopus subject areas
- Instrumentation
- Nuclear and High Energy Physics
Cite this
Quantitative metabolism using AMS : Choosing a labeled precursor. / Links, Jennifer; Palmblad, Magnus; Ognibene, Ted; Turteltaub, Ken W; Bench, Graham.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 268, No. 7-8, 04.2010, p. 1309-1312.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative metabolism using AMS
T2 - Choosing a labeled precursor
AU - Links, Jennifer
AU - Palmblad, Magnus
AU - Ognibene, Ted
AU - Turteltaub, Ken W
AU - Bench, Graham
PY - 2010/4
Y1 - 2010/4
N2 - Biological radioisotope studies suffer from a lack of sensitive measurement techniques and therefore traditionally require large amounts of labeled material to produce a measurable signal. Such quantities of materials are often significantly higher than naturally-occurring levels preventing these studies from replicating physiological conditions. AMS affords the sensitivity necessary to perform biological radioisotope studies with low levels of labeled material that preserve physiological conditions. The choice of labeled material can substantially affect the ease of interpretation and comprehensiveness of these studies. Here, the benefits and limitations of whole-cell labeling with 14C-glucose and targeted pathway labeling with 14C-nicotinic acid are discussed and compared.
AB - Biological radioisotope studies suffer from a lack of sensitive measurement techniques and therefore traditionally require large amounts of labeled material to produce a measurable signal. Such quantities of materials are often significantly higher than naturally-occurring levels preventing these studies from replicating physiological conditions. AMS affords the sensitivity necessary to perform biological radioisotope studies with low levels of labeled material that preserve physiological conditions. The choice of labeled material can substantially affect the ease of interpretation and comprehensiveness of these studies. Here, the benefits and limitations of whole-cell labeling with 14C-glucose and targeted pathway labeling with 14C-nicotinic acid are discussed and compared.
KW - AMS
KW - Biology
KW - Cell labeling
KW - Glucose
KW - HPLC
KW - Metabolism
KW - NAD
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=77949908208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949908208&partnerID=8YFLogxK
U2 - 10.1016/j.nimb.2009.10.160
DO - 10.1016/j.nimb.2009.10.160
M3 - Article
AN - SCOPUS:77949908208
VL - 268
SP - 1309
EP - 1312
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
SN - 0168-583X
IS - 7-8
ER -