Quantitative metabolism using AMS: Choosing a labeled precursor

Jennifer Links, Magnus Palmblad, Ted Ognibene, Ken W Turteltaub, Graham Bench

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Biological radioisotope studies suffer from a lack of sensitive measurement techniques and therefore traditionally require large amounts of labeled material to produce a measurable signal. Such quantities of materials are often significantly higher than naturally-occurring levels preventing these studies from replicating physiological conditions. AMS affords the sensitivity necessary to perform biological radioisotope studies with low levels of labeled material that preserve physiological conditions. The choice of labeled material can substantially affect the ease of interpretation and comprehensiveness of these studies. Here, the benefits and limitations of whole-cell labeling with 14C-glucose and targeted pathway labeling with 14C-nicotinic acid are discussed and compared.

Original languageEnglish (US)
Pages (from-to)1309-1312
Number of pages4
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume268
Issue number7-8
DOIs
StatePublished - Apr 2010
Externally publishedYes

Keywords

  • AMS
  • Biology
  • Cell labeling
  • Glucose
  • HPLC
  • Metabolism
  • NAD
  • Yeast

ASJC Scopus subject areas

  • Instrumentation
  • Nuclear and High Energy Physics

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