Quantitative measurement of anthrax toxin receptor messenger RNA in primary mononuclear phagocytes

Christopher Premanandan, Michael Dale Lairmore, Soledad Fernandez, Andrew J. Phipps

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Two anthrax toxin receptors have been identified, tumor endothelial marker 8 (TEM8) and capillary morphogenesis protein 2 (CMG2). Both receptors have been shown to be capable of facilitating the entry of Bacillus anthracis exotoxins into the cytosol of susceptible cells. The levels of TEM8 and CMG2 RNA transcripts present in human primary macrophages derived from multiple unrelated donors and primary mouse macrophages have not been well described. In this communication, we examined the expression of mRNA transcripts of TEM8 and CMG2 in primary human and mouse macrophages and mouse tissues by standard and quantitative real-time RT-PCR. Our results indicate that CMG2 transcripts are preferentially expressed over TEM8 transcripts in primary human and mouse macrophages as compared to immortalized cell lines.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalMicrobial Pathogenesis
Volume41
Issue number4-5
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Phagocytes
Tumor Biomarkers
Morphogenesis
Macrophages
Messenger RNA
Proteins
Bacillus anthracis
Exotoxins
Unrelated Donors
Cytosol
Real-Time Polymerase Chain Reaction
RNA
Cell Line
anthrax toxin receptors

Keywords

  • Anthrax
  • CMG2
  • Macrophage
  • Receptor
  • TEM8

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Quantitative measurement of anthrax toxin receptor messenger RNA in primary mononuclear phagocytes. / Premanandan, Christopher; Lairmore, Michael Dale; Fernandez, Soledad; Phipps, Andrew J.

In: Microbial Pathogenesis, Vol. 41, No. 4-5, 10.2006, p. 193-198.

Research output: Contribution to journalArticle

Premanandan, Christopher ; Lairmore, Michael Dale ; Fernandez, Soledad ; Phipps, Andrew J. / Quantitative measurement of anthrax toxin receptor messenger RNA in primary mononuclear phagocytes. In: Microbial Pathogenesis. 2006 ; Vol. 41, No. 4-5. pp. 193-198.
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