Quantitative histology and cytochrome P-450 immunocytochemistry of the lung parenchyma following 6 months of exposure of strain A/J mice to cigarette sidestream smoke

Kent E Pinkerton, Janice L. Peake, Imelda Espiritu, Michael Goldsmith, Hanspeter Witschi

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Abstract

Male strain A/l mice were exposed for 6 h/day, 5 days/wk to aged and diluted cigarette sidestream smoke (ADSS) at a chamber concentration of 4 mg/m3 of total suspended particulate matter (TSP). After 6 mo, the lungs were examined for altered expression of cytochrome P-450 isozymes and for differences in total alveolar tissue volume or surface area, as well as changes in the numbers of epithelial type II cells and alveolar macrophages. Morphologic measurements showed no statistically significant differences for the air, alveolar tissue, or capillary volumes of the lungs or changes in the total number of epithelial type II cells or alveolar macrophages. In contrast, cytochrome P-4501A1 was elevated in the lungs of ADSS-exposed animals and localized in capillary endothelial cells. CYP2B1 was present in airway epithelial cells as well as in epithelial cells throughout the lung parenchyma, but its distribution was not changed by ADSS exposure. Isozyme CYP2E1 was also found in airway epithelial cells, but not in the lung parenchyma, with no differences noted between ADSS exposed animals and controls. CYP2F2 was found in the bronchiolar Clara cells and in type II cells located within the alveolar parenchyma, but was also unchanged. It is concluded that chronic exposure to cigarette ADSS at 4 mg/m3 of TSP produces no changes in alveolar macrophages or epithelial type II cells in mouse lung but increases the expression of cytochrome P4501A1.

Original languageEnglish (US)
Pages (from-to)927-945
Number of pages19
JournalInhalation Toxicology
Volume8
Issue number9
StatePublished - Dec 1996

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Histology
Smoke
Tobacco Products
Cytochrome P-450 Enzyme System
Epithelial Cells
Immunohistochemistry
Lung
Alveolar Macrophages
Particulate Matter
Cytochromes
Isoenzymes
Animals
Cytochrome P-450 CYP2B1
Tissue
Cytochrome P-450 CYP2E1
Endothelial cells
Endothelial Cells
Air

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Quantitative histology and cytochrome P-450 immunocytochemistry of the lung parenchyma following 6 months of exposure of strain A/J mice to cigarette sidestream smoke. / Pinkerton, Kent E; Peake, Janice L.; Espiritu, Imelda; Goldsmith, Michael; Witschi, Hanspeter.

In: Inhalation Toxicology, Vol. 8, No. 9, 12.1996, p. 927-945.

Research output: Contribution to journalArticle

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