Quantitative genetic interaction mapping using the E-Map approach

Sean Collins, Assen Roguev, Nevan J. Krogan

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Genetic interactions represent the degree to which the presence of one mutation modulates the phenotype of a second mutation. In recent years, approaches for measuring genetic interactions systematically and quantitatively have proven to be effective tools for unbiased characterization of gene function and have provided valuable data for analyses of evolution. Here, we present protocols for systematic measurement of genetic interactions with respect to organismal growth rate for two yeast species.

Original languageEnglish (US)
Pages (from-to)205-231
Number of pages27
JournalMethods in Enzymology
Volume470
Issue numberC
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

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Yeast
Genes
Mutation
Yeasts
Phenotype
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Quantitative genetic interaction mapping using the E-Map approach. / Collins, Sean; Roguev, Assen; Krogan, Nevan J.

In: Methods in Enzymology, Vol. 470, No. C, 01.01.2010, p. 205-231.

Research output: Contribution to journalArticle

Collins, Sean ; Roguev, Assen ; Krogan, Nevan J. / Quantitative genetic interaction mapping using the E-Map approach. In: Methods in Enzymology. 2010 ; Vol. 470, No. C. pp. 205-231.
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