Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles

Ahjeong Son, Ian M. Kennedy, Kate M. Scow, Krassimira R. Hristova

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A magnetic/luminescent nanoparticles (MLNPs) based DNA hybridization method was developed for quantitative monitoring of antibiotic resistance genes and gene-expression in environmental samples. Manipulation of magnetic field enabled the separation of the MLNPs-DNA hybrids from the solution and the fluorescence of MLNPs normalized the quantity of target DNA. In our newly developed MLNPs-DNA assay, linear standard curves (R2 = 0.99) of target gene was determined with the detection limit of 620 gene copies. The potential risk of increased bacterial antibiotic resistance was assessed by quantitative monitoring of tetracycline resistance (i.e., tetQ gene) in wastewater microcosms. The gene abundance and its expression showed a significant increase of tetQ gene copies with the addition of tetracycline, triclosan (TCS), or triclocarban (TCC). A real-time PCR assay was employed to verify the quantification capability of the MLNPs-DNA assay and accordingly both assays have shown strong correlation (R2 = 0.93). This non-PCR based MLNPs-DNA assay has demonstrated its potential for gene quantification via a rapid, simple, and high throughput platform and its novel use of internal calibration standards.

Original languageEnglish (US)
Pages (from-to)1362-1367
Number of pages6
JournalJournal of Environmental Monitoring
Volume12
Issue number6
DOIs
StatePublished - 2010

Fingerprint

Microbial Genes
Tetracycline Resistance
Tetracycline
Nanoparticles
Genes
Assays
Monitoring
gene
DNA
monitoring
assay
antibiotic resistance
Bacterial Drug Resistance
Triclosan
Anti-Bacterial Agents
Magnetic Fields
Microbial Drug Resistance
Waste Water
nanoparticle
Gene expression

ASJC Scopus subject areas

  • Management, Monitoring, Policy and Law
  • Public Health, Environmental and Occupational Health

Cite this

Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles. / Son, Ahjeong; Kennedy, Ian M.; Scow, Kate M.; Hristova, Krassimira R.

In: Journal of Environmental Monitoring, Vol. 12, No. 6, 2010, p. 1362-1367.

Research output: Contribution to journalArticle

Son, Ahjeong ; Kennedy, Ian M. ; Scow, Kate M. ; Hristova, Krassimira R. / Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles. In: Journal of Environmental Monitoring. 2010 ; Vol. 12, No. 6. pp. 1362-1367.
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