Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography

Alan R Buckpitt, D. E. Rollins, S. D. Nelson, R. B. Franklin, J. R. Mitchell

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-14C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.

Original languageEnglish (US)
Pages (from-to)168-177
Number of pages10
JournalAnalytical Biochemistry
Volume83
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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