Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography

Alan R Buckpitt; D. E. Rollins; S. D. Nelson; R. B. Franklin; J. R. Mitchell

doi:10.1016/0003-2697(77)90522-X

Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography

Alan R Buckpitt, D. E. Rollins, S. D. Nelson, R. B. Franklin, J. R. Mitchell

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-¹⁴C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.

Original language	English (US)
Pages (from-to)	168-177
Number of pages	10
Journal	Analytical Biochemistry
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(77)90522-X
State	Published - 1977
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography",

abstract = "A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-14C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.",

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year = "1977",

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T1 - Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography

AU - Buckpitt, Alan R

AU - Rollins, D. E.

AU - Nelson, S. D.

AU - Franklin, R. B.

AU - Mitchell, J. R.

PY - 1977

Y1 - 1977

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AB - A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-14C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.

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