Abstract
A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-14C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 168-177 |
| Number of pages | 10 |
| Journal | Analytical Biochemistry |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1977 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Fingerprint Dive into the research topics of 'Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography'. Together they form a unique fingerprint.
Cite this
Buckpitt, A. R., Rollins, D. E., Nelson, S. D., Franklin, R. B., & Mitchell, J. R. (1977). Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography. Analytical Biochemistry, 83(1), 168-177. https://doi.org/10.1016/0003-2697(77)90522-X