Quantitative determination of ginsenosides by high-performance liquid chromatography-tandem mass spectrometry

Qin C. Ji, Martha R. Harkey, Gary L. Henderson, M. Eric Gershwin, Judith S. Stern, Robert M. Hackman

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

An HPLC-MS/MS method was developed for the quantitative determination of ginsenosides, which are the marker compounds for herbal products containing Panax ginseng (Korean or Chinese ginseng) and P. quinquefolius (American ginseng). Samples were extracted with BondElut® C18 HF extraction columns and the concentrations of seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) were determined by reversed-phase HPLC-MS/MS employing a quadrupole-ion trap mass spectrometer. Both positive and negative electrospray ionisation techniques were evaluated. Positive ionisation spectra of these compounds gave strong sodium adduct molecular and sodium adduct dimer ions. Negative ionisation yielded the molecular ion primarily and was, therefore, used for analysis: quantitative determination was based on the most abundant product ions for each ginsenoside. The method was used to extract and analyse commercial samples of P. ginseng and P. quinquefolius.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalPhytochemical Analysis
Volume12
Issue number5
DOIs
StatePublished - 2001

Fingerprint

Ginsenosides
Panax
High performance liquid chromatography
Tandem Mass Spectrometry
Mass spectrometry
high performance liquid chromatography
High Pressure Liquid Chromatography
Ions
ionization
ions
Panax quinquefolius
Panax ginseng
Ionization
Sodium
sodium
Electrospray ionization
reversed-phase high performance liquid chromatography
Mass spectrometers
spectrometers
Dimers

Keywords

  • Ginseng
  • Ginsenosides
  • High performance liquid chromatography-mass spectrometry
  • Panax ginseng
  • Panax quinquefolius

ASJC Scopus subject areas

  • Plant Science
  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Quantitative determination of ginsenosides by high-performance liquid chromatography-tandem mass spectrometry. / Ji, Qin C.; Harkey, Martha R.; Henderson, Gary L.; Gershwin, M. Eric; Stern, Judith S.; Hackman, Robert M.

In: Phytochemical Analysis, Vol. 12, No. 5, 2001, p. 320-326.

Research output: Contribution to journalArticle

Ji, Qin C. ; Harkey, Martha R. ; Henderson, Gary L. ; Gershwin, M. Eric ; Stern, Judith S. ; Hackman, Robert M. / Quantitative determination of ginsenosides by high-performance liquid chromatography-tandem mass spectrometry. In: Phytochemical Analysis. 2001 ; Vol. 12, No. 5. pp. 320-326.
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