Quantitative determination of carisoprodol and its metabolites in equine urine and serum by liquid chromatography-tandem mass spectrometry

W. Skinner, D. McKemie, Scott D Stanley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of carisoprodol and its metabolites, meprobamate and hydroxycarisoprodol, in equine urine and serum. The method consists of solid phase extraction followed by LC-MS-MS using electrospray ionization (ESI) and multiple reaction monitoring. Validation of the method showed excellent sensitivity, selectivity, accuracy, precision and ruggedness. The urinary excretion and serum profiles of these analytes were determined after oral administration of carisoprodol and meprobamate. The determination of hydroxycarisoprodol in urine and/or intact carisprodol in serum allows one to effectively distinguish between specimens arising from administration of carisoprodol (a class 4 drug violation) and meprobamate (a class 2 violation).

Original languageEnglish (US)
JournalChromatographia
Volume59
Issue numberSUPPL.
DOIs
StatePublished - 2004

Fingerprint

Meprobamate
Liquid chromatography
Carisoprodol
Metabolites
Tandem Mass Spectrometry
Liquid Chromatography
Horses
Mass spectrometry
Urine
Serum
Electrospray ionization
Solid Phase Extraction
Oral Administration
Monitoring
Pharmaceutical Preparations

Keywords

  • Carisoprodol
  • Column liquid chromatography-mass spectrometry
  • Meprobamate
  • Urinary elimination

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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abstract = "A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of carisoprodol and its metabolites, meprobamate and hydroxycarisoprodol, in equine urine and serum. The method consists of solid phase extraction followed by LC-MS-MS using electrospray ionization (ESI) and multiple reaction monitoring. Validation of the method showed excellent sensitivity, selectivity, accuracy, precision and ruggedness. The urinary excretion and serum profiles of these analytes were determined after oral administration of carisoprodol and meprobamate. The determination of hydroxycarisoprodol in urine and/or intact carisprodol in serum allows one to effectively distinguish between specimens arising from administration of carisoprodol (a class 4 drug violation) and meprobamate (a class 2 violation).",
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AU - Stanley, Scott D

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N2 - A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of carisoprodol and its metabolites, meprobamate and hydroxycarisoprodol, in equine urine and serum. The method consists of solid phase extraction followed by LC-MS-MS using electrospray ionization (ESI) and multiple reaction monitoring. Validation of the method showed excellent sensitivity, selectivity, accuracy, precision and ruggedness. The urinary excretion and serum profiles of these analytes were determined after oral administration of carisoprodol and meprobamate. The determination of hydroxycarisoprodol in urine and/or intact carisprodol in serum allows one to effectively distinguish between specimens arising from administration of carisoprodol (a class 4 drug violation) and meprobamate (a class 2 violation).

AB - A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of carisoprodol and its metabolites, meprobamate and hydroxycarisoprodol, in equine urine and serum. The method consists of solid phase extraction followed by LC-MS-MS using electrospray ionization (ESI) and multiple reaction monitoring. Validation of the method showed excellent sensitivity, selectivity, accuracy, precision and ruggedness. The urinary excretion and serum profiles of these analytes were determined after oral administration of carisoprodol and meprobamate. The determination of hydroxycarisoprodol in urine and/or intact carisprodol in serum allows one to effectively distinguish between specimens arising from administration of carisoprodol (a class 4 drug violation) and meprobamate (a class 2 violation).

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