Quantitative cerebral blood flow with optical coherence tomography

Vivek Srinivasan, Sava Sakadžić, Iwona Gorczynska, Svetlana Ruvinskaya, Weicheng Wu, James G. Fujimoto, David A. Boas

Research output: Contribution to journalArticle

187 Scopus citations

Abstract

Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and cranial window preparations, we present methods and algorithms for regional CBF measurements in the rat cortex. Towards this end, we develop and validate a quantitative statistical model to describe the effect of static tissue on velocity sensitivity. This model is used to design scanning protocols and algorithms for sensitive 3D flow measurements and angiography of the cortex. We also introduce a method of absolute flow calculation that does not require explicit knowledge of vessel angles. We show that OCT estimates of absolute CBF values in rats agree with prior measures by autoradiography, suggesting that Doppler OCT can perform absolute flow measurements in animal models.

Original languageEnglish (US)
Pages (from-to)2477-2494
Number of pages18
JournalOptics Express
Volume18
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Srinivasan, V., Sakadžić, S., Gorczynska, I., Ruvinskaya, S., Wu, W., Fujimoto, J. G., & Boas, D. A. (2010). Quantitative cerebral blood flow with optical coherence tomography. Optics Express, 18(3), 2477-2494. https://doi.org/10.1364/OE.18.002477