Abstract
A highly sensitive and precise high-performance liquid chromatography (HPLC) assay was developed and validated for the quantitation of Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl) phenoxy]ethanol (FC-1271a) in human plasma. Plasma samples (1.0 ml) containing FC-1271a and internal standard (toremifene citrate; Fareston(®)) were extracted using a 2% 1-butanol, 98% hexane solution with an extraction efficiency of >97%. Samples were reconstituted in methanol, irradiated with high intensity ultraviolet light (254 nm) for 1 min, and injected onto a C18 reverse phase column. Samples were eluted isocratically at a flow-rate of 0.5 ml/min with a mobile phase consisting of 6.5% water and 0.5% triethylamine in methanol. The fluorescence of photochemically activated compounds was detected using a fluorometer set at an excitation wavelength of 266 nm and emission wavelength of 370 nm. Under these assay conditions, standard calibration curves were linear through a concentration range of 10-400 ng/ml. In summary, we have developed and validated an HPLC assay to quantitate FC-1271a in human plasma. Copyright (C) 1999 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 163-171 |
| Number of pages | 9 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 724 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 5 1999 |
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Keywords
- FC-1271a
- Triphenylethylene
- Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl)phenoxy]ethanol
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Quantitative analysis of Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl)phenoxy]ethanol in human plasma using high-performance liquid chromatography. / Taras, Tracy L.; Wurz, Gregory T.; Hellmann-Blumberg, Utha; DeGregorio, Michael W.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 724, No. 1, 05.03.1999, p. 163-171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative analysis of Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl)phenoxy]ethanol in human plasma using high-performance liquid chromatography
AU - Taras, Tracy L.
AU - Wurz, Gregory T.
AU - Hellmann-Blumberg, Utha
AU - DeGregorio, Michael W.
PY - 1999/3/5
Y1 - 1999/3/5
N2 - A highly sensitive and precise high-performance liquid chromatography (HPLC) assay was developed and validated for the quantitation of Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl) phenoxy]ethanol (FC-1271a) in human plasma. Plasma samples (1.0 ml) containing FC-1271a and internal standard (toremifene citrate; Fareston(®)) were extracted using a 2% 1-butanol, 98% hexane solution with an extraction efficiency of >97%. Samples were reconstituted in methanol, irradiated with high intensity ultraviolet light (254 nm) for 1 min, and injected onto a C18 reverse phase column. Samples were eluted isocratically at a flow-rate of 0.5 ml/min with a mobile phase consisting of 6.5% water and 0.5% triethylamine in methanol. The fluorescence of photochemically activated compounds was detected using a fluorometer set at an excitation wavelength of 266 nm and emission wavelength of 370 nm. Under these assay conditions, standard calibration curves were linear through a concentration range of 10-400 ng/ml. In summary, we have developed and validated an HPLC assay to quantitate FC-1271a in human plasma. Copyright (C) 1999 Elsevier Science B.V.
AB - A highly sensitive and precise high-performance liquid chromatography (HPLC) assay was developed and validated for the quantitation of Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl) phenoxy]ethanol (FC-1271a) in human plasma. Plasma samples (1.0 ml) containing FC-1271a and internal standard (toremifene citrate; Fareston(®)) were extracted using a 2% 1-butanol, 98% hexane solution with an extraction efficiency of >97%. Samples were reconstituted in methanol, irradiated with high intensity ultraviolet light (254 nm) for 1 min, and injected onto a C18 reverse phase column. Samples were eluted isocratically at a flow-rate of 0.5 ml/min with a mobile phase consisting of 6.5% water and 0.5% triethylamine in methanol. The fluorescence of photochemically activated compounds was detected using a fluorometer set at an excitation wavelength of 266 nm and emission wavelength of 370 nm. Under these assay conditions, standard calibration curves were linear through a concentration range of 10-400 ng/ml. In summary, we have developed and validated an HPLC assay to quantitate FC-1271a in human plasma. Copyright (C) 1999 Elsevier Science B.V.
KW - FC-1271a
KW - Triphenylethylene
KW - Z-2-[4-(4-chloro-1,2-diphenyl-but-1-enyl)phenoxy]ethanol
UR - http://www.scopus.com/inward/record.url?scp=0032970908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032970908&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(98)00571-4
DO - 10.1016/S0378-4347(98)00571-4
M3 - Article
VL - 724
SP - 163
EP - 171
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 1
ER -