Quantitative analysis of hepatitis C virus in peripheral blood and liver: Replication detected only in liver

J. Boisvert, Xiaosong He, R. Cheung, E. B. Keeffe, T. Wright, H. B. Greenberg

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Prior studies seeking evidence of viral replication in peripheral lymphocytes of hepatitis C virus (HCV)-infected patients have yielded conflicting results. This study sought to quantitatively determine whether a permissive HCV cell interaction could be detected in leukocytes from infected patients. Peripheral leukocytes from chronically infected patients were purified and were tested for HCV RNA. The results show that virus load is highest in B cells. Other subsets of peripheral leukocytes consistently had very low levels of viral RNA or were negative. Negative-strand HCV was found only in hepatocytes. To determine whether HCV replication could be induced by activation, B cells from HCV-infected patients were stimulated in vitro. No HCV replicating in peripheral leukocytes was detected by a highly sensitive assay. If HCV replication occurs in the leukocyte subsets analyzed here, it is at extremely low levels or occurs under alternate physiological conditions.

Original languageEnglish (US)
Pages (from-to)827-835
Number of pages9
JournalJournal of Infectious Diseases
Volume184
Issue number7
DOIs
StatePublished - Oct 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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