Quantitative analysis of angiogenesis using confocal laser scanning microscopy

Linlang Guo, Patricia Burke, Su Hao Lo, Regina F Gandour-Edwards, Derick H Lau

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Angiogenesis is essential for tumor growth and metastasis. Angiogenesis is commonly quantified by measuring microvessel density (MVD) within tumors. In this report, we compared light microscopy with confocal laser scanning microscopy (CLSM) in the qualitative and quantitative analysis of angiogenesis. MVDs were determined manually in a lung tumor xenograft and a normal skeletal muscle using CD31 immunohistochemical staining and light microscopy. Area of three-dimensional representation of microvessels, detected as CD31 immunofluorescence, was measured automatically using computer-assisted CLSM. By manual counting under light microscopy, the relative level of MVD of the lung tumor vs. skeletal muscle was 0.8. However, the corresponding relative level of microvessels was 3.4 as determined by computer-assisted CLSM. Futhermore, the architecture of microvessels was better delineated with CLSM than with light microscopy. We have applied this CLSM method for analyzing the antiangiogenic effect of an anticancer drug, paclitaxel, in the lung tumor xenograft model. We conclude that CLSM is an appropriate method for quantitative and qualitative analysis of microvasculature in normal and tumor tissues.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalAngiogenesis
Volume4
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Microvessels
Confocal Microscopy
Tumors
Microscopic examination
Scanning
Optical microscopy
Lasers
Microscopy
Chemical analysis
Light
Neoplasms
Heterografts
Lung
Muscle
Skeletal Muscle
Paclitaxel
Fluorescent Antibody Technique
Tissue
Staining and Labeling
Neoplasm Metastasis

Keywords

  • Angiogenesis quantification
  • Confocal laser scanning microscopy

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Clinical Biochemistry
  • Polymers and Plastics

Cite this

Quantitative analysis of angiogenesis using confocal laser scanning microscopy. / Guo, Linlang; Burke, Patricia; Lo, Su Hao; Gandour-Edwards, Regina F; Lau, Derick H.

In: Angiogenesis, Vol. 4, No. 3, 2001, p. 187-191.

Research output: Contribution to journalArticle

@article{96bcfdfb0cae4597b90107efc6fc3af6,
title = "Quantitative analysis of angiogenesis using confocal laser scanning microscopy",
abstract = "Angiogenesis is essential for tumor growth and metastasis. Angiogenesis is commonly quantified by measuring microvessel density (MVD) within tumors. In this report, we compared light microscopy with confocal laser scanning microscopy (CLSM) in the qualitative and quantitative analysis of angiogenesis. MVDs were determined manually in a lung tumor xenograft and a normal skeletal muscle using CD31 immunohistochemical staining and light microscopy. Area of three-dimensional representation of microvessels, detected as CD31 immunofluorescence, was measured automatically using computer-assisted CLSM. By manual counting under light microscopy, the relative level of MVD of the lung tumor vs. skeletal muscle was 0.8. However, the corresponding relative level of microvessels was 3.4 as determined by computer-assisted CLSM. Futhermore, the architecture of microvessels was better delineated with CLSM than with light microscopy. We have applied this CLSM method for analyzing the antiangiogenic effect of an anticancer drug, paclitaxel, in the lung tumor xenograft model. We conclude that CLSM is an appropriate method for quantitative and qualitative analysis of microvasculature in normal and tumor tissues.",
keywords = "Angiogenesis quantification, Confocal laser scanning microscopy",
author = "Linlang Guo and Patricia Burke and Lo, {Su Hao} and Gandour-Edwards, {Regina F} and Lau, {Derick H}",
year = "2001",
doi = "10.1023/A:1014010801754",
language = "English (US)",
volume = "4",
pages = "187--191",
journal = "Angiogenesis",
issn = "0969-6970",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Quantitative analysis of angiogenesis using confocal laser scanning microscopy

AU - Guo, Linlang

AU - Burke, Patricia

AU - Lo, Su Hao

AU - Gandour-Edwards, Regina F

AU - Lau, Derick H

PY - 2001

Y1 - 2001

N2 - Angiogenesis is essential for tumor growth and metastasis. Angiogenesis is commonly quantified by measuring microvessel density (MVD) within tumors. In this report, we compared light microscopy with confocal laser scanning microscopy (CLSM) in the qualitative and quantitative analysis of angiogenesis. MVDs were determined manually in a lung tumor xenograft and a normal skeletal muscle using CD31 immunohistochemical staining and light microscopy. Area of three-dimensional representation of microvessels, detected as CD31 immunofluorescence, was measured automatically using computer-assisted CLSM. By manual counting under light microscopy, the relative level of MVD of the lung tumor vs. skeletal muscle was 0.8. However, the corresponding relative level of microvessels was 3.4 as determined by computer-assisted CLSM. Futhermore, the architecture of microvessels was better delineated with CLSM than with light microscopy. We have applied this CLSM method for analyzing the antiangiogenic effect of an anticancer drug, paclitaxel, in the lung tumor xenograft model. We conclude that CLSM is an appropriate method for quantitative and qualitative analysis of microvasculature in normal and tumor tissues.

AB - Angiogenesis is essential for tumor growth and metastasis. Angiogenesis is commonly quantified by measuring microvessel density (MVD) within tumors. In this report, we compared light microscopy with confocal laser scanning microscopy (CLSM) in the qualitative and quantitative analysis of angiogenesis. MVDs were determined manually in a lung tumor xenograft and a normal skeletal muscle using CD31 immunohistochemical staining and light microscopy. Area of three-dimensional representation of microvessels, detected as CD31 immunofluorescence, was measured automatically using computer-assisted CLSM. By manual counting under light microscopy, the relative level of MVD of the lung tumor vs. skeletal muscle was 0.8. However, the corresponding relative level of microvessels was 3.4 as determined by computer-assisted CLSM. Futhermore, the architecture of microvessels was better delineated with CLSM than with light microscopy. We have applied this CLSM method for analyzing the antiangiogenic effect of an anticancer drug, paclitaxel, in the lung tumor xenograft model. We conclude that CLSM is an appropriate method for quantitative and qualitative analysis of microvasculature in normal and tumor tissues.

KW - Angiogenesis quantification

KW - Confocal laser scanning microscopy

UR - http://www.scopus.com/inward/record.url?scp=0035715329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035715329&partnerID=8YFLogxK

U2 - 10.1023/A:1014010801754

DO - 10.1023/A:1014010801754

M3 - Article

C2 - 11911016

AN - SCOPUS:0035715329

VL - 4

SP - 187

EP - 191

JO - Angiogenesis

JF - Angiogenesis

SN - 0969-6970

IS - 3

ER -