Quantitative analysis of an anaphase B switch

Predicted role for a microtubule catastrophe gradient

Dhanya K. Cheerambathur, Gul Civelekoglu-Scholey, Ingrid Brust-Mascher, Patrizia Sommi, Alex Mogilner, Jonathan M. Scholey

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Anaphase B in Drosophila embryos is initiated by the inhibition of microtubule (MT) depolymerization at spindle poles, which allows outwardly sliding interpolar (ip) MTs to drive pole-pole separation. Using fluorescence recovery after photobleaching, we observed that MTs throughout the preanaphase B spindle are very dynamic and display complete recovery of fluorescence, but during anaphase B, MTs proximal to the poles stabilize and therefore display lower recovery than those elsewhere. Fluorescence microscopy of the MT tip tracker EB1 revealed that growing MT plus ends localize throughout the preanaphase B spindle but concentrate in the overlap region of interpolar MTs (ipMTs) at anaphase B onset. None of these changes occurred in the presence of nondegradable cyclin B. Modeling suggests that they depend on the establishment of a spatial gradient of MT plus-end catastrophe frequencies, decreasing toward the equator. The resulting redistribution of ipMT plus ends to the overlap zone, together with the suppression of minus-end depolymerization at the poles, could constitute a mechanical switch that initiates spindle elongation.

Original languageEnglish (US)
Pages (from-to)995-1004
Number of pages10
JournalJournal of Cell Biology
Volume177
Issue number6
DOIs
StatePublished - Jun 18 2007

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Anaphase
Microtubules
Spindle Poles
Fluorescence Recovery After Photobleaching
Cyclin B
Fluorescence Microscopy
Drosophila
Embryonic Structures
Fluorescence

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cheerambathur, D. K., Civelekoglu-Scholey, G., Brust-Mascher, I., Sommi, P., Mogilner, A., & Scholey, J. M. (2007). Quantitative analysis of an anaphase B switch: Predicted role for a microtubule catastrophe gradient. Journal of Cell Biology, 177(6), 995-1004. https://doi.org/10.1083/jcb.200611113

Quantitative analysis of an anaphase B switch : Predicted role for a microtubule catastrophe gradient. / Cheerambathur, Dhanya K.; Civelekoglu-Scholey, Gul; Brust-Mascher, Ingrid; Sommi, Patrizia; Mogilner, Alex; Scholey, Jonathan M.

In: Journal of Cell Biology, Vol. 177, No. 6, 18.06.2007, p. 995-1004.

Research output: Contribution to journalArticle

Cheerambathur, DK, Civelekoglu-Scholey, G, Brust-Mascher, I, Sommi, P, Mogilner, A & Scholey, JM 2007, 'Quantitative analysis of an anaphase B switch: Predicted role for a microtubule catastrophe gradient', Journal of Cell Biology, vol. 177, no. 6, pp. 995-1004. https://doi.org/10.1083/jcb.200611113
Cheerambathur DK, Civelekoglu-Scholey G, Brust-Mascher I, Sommi P, Mogilner A, Scholey JM. Quantitative analysis of an anaphase B switch: Predicted role for a microtubule catastrophe gradient. Journal of Cell Biology. 2007 Jun 18;177(6):995-1004. https://doi.org/10.1083/jcb.200611113
Cheerambathur, Dhanya K. ; Civelekoglu-Scholey, Gul ; Brust-Mascher, Ingrid ; Sommi, Patrizia ; Mogilner, Alex ; Scholey, Jonathan M. / Quantitative analysis of an anaphase B switch : Predicted role for a microtubule catastrophe gradient. In: Journal of Cell Biology. 2007 ; Vol. 177, No. 6. pp. 995-1004.
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