Abstract
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1743-1747 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 323 |
| Issue number | 5922 |
| DOIs | |
| State | Published - Mar 27 2009 |
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Cite this
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. / Hübner, Wolfgang; McNerney, Gregory P.; Chen, Ping; Dale, Benjamin M.; Gordon, Ronald E.; Chuang, Frank; Li, Xiao Dong; Asmuth, David; Huser, Thomas R; Chen, Benjamin K.
In: Science, Vol. 323, No. 5922, 27.03.2009, p. 1743-1747.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative 3D video microscopy of HIV transfer across T cell virological synapses
AU - Hübner, Wolfgang
AU - McNerney, Gregory P.
AU - Chen, Ping
AU - Dale, Benjamin M.
AU - Gordon, Ronald E.
AU - Chuang, Frank
AU - Li, Xiao Dong
AU - Asmuth, David
AU - Huser, Thomas R
AU - Chen, Benjamin K.
PY - 2009/3/27
Y1 - 2009/3/27
N2 - The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
AB - The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
UR - http://www.scopus.com/inward/record.url?scp=63449126562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63449126562&partnerID=8YFLogxK
U2 - 10.1126/science.1167525
DO - 10.1126/science.1167525
M3 - Article
C2 - 19325119
AN - SCOPUS:63449126562
VL - 323
SP - 1743
EP - 1747
JO - Science
JF - Science
SN - 0036-8075
IS - 5922
ER -