Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Wolfgang Hübner, Gregory P. McNerney, Ping Chen, Benjamin M. Dale, Ronald E. Gordon, Frank Chuang, Xiao Dong Li, David Asmuth, Thomas R Huser, Benjamin K. Chen

Research output: Contribution to journalArticle

292 Citations (Scopus)

Abstract

The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

Original languageEnglish (US)
Pages (from-to)1743-1747
Number of pages5
JournalScience
Volume323
Issue number5922
DOIs
StatePublished - Mar 27 2009

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Video Microscopy
Synapses
HIV
T-Lymphocytes
Cell Adhesion
gag Gene Products
Endocytosis
Electron Microscopy
Clone Cells
Viruses
Infection

ASJC Scopus subject areas

  • General

Cite this

Hübner, W., McNerney, G. P., Chen, P., Dale, B. M., Gordon, R. E., Chuang, F., ... Chen, B. K. (2009). Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science, 323(5922), 1743-1747. https://doi.org/10.1126/science.1167525

Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. / Hübner, Wolfgang; McNerney, Gregory P.; Chen, Ping; Dale, Benjamin M.; Gordon, Ronald E.; Chuang, Frank; Li, Xiao Dong; Asmuth, David; Huser, Thomas R; Chen, Benjamin K.

In: Science, Vol. 323, No. 5922, 27.03.2009, p. 1743-1747.

Research output: Contribution to journalArticle

Hübner, W, McNerney, GP, Chen, P, Dale, BM, Gordon, RE, Chuang, F, Li, XD, Asmuth, D, Huser, TR & Chen, BK 2009, 'Quantitative 3D video microscopy of HIV transfer across T cell virological synapses', Science, vol. 323, no. 5922, pp. 1743-1747. https://doi.org/10.1126/science.1167525
Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang F et al. Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science. 2009 Mar 27;323(5922):1743-1747. https://doi.org/10.1126/science.1167525
Hübner, Wolfgang ; McNerney, Gregory P. ; Chen, Ping ; Dale, Benjamin M. ; Gordon, Ronald E. ; Chuang, Frank ; Li, Xiao Dong ; Asmuth, David ; Huser, Thomas R ; Chen, Benjamin K. / Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. In: Science. 2009 ; Vol. 323, No. 5922. pp. 1743-1747.
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