Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Wolfgang Hübner; Gregory P. McNerney; Ping Chen; Benjamin M. Dale; Ronald E. Gordon; Frank Chuang; Xiao Dong Li; David Asmuth; Thomas R Huser; Benjamin K. Chen

doi:10.1126/science.1167525

Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Wolfgang Hübner, Gregory P. McNerney, Ping Chen, Benjamin M. Dale, Ronald E. Gordon, Frank Chuang, Xiao Dong Li, David Asmuth, Thomas R Huser, Benjamin K. Chen

Research output: Contribution to journal › Article › peer-review

342 Scopus citations

Abstract

The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

Original language	English (US)
Pages (from-to)	1743-1747
Number of pages	5
Journal	Science
Volume	323
Issue number	5922
DOIs	https://doi.org/10.1126/science.1167525
State	Published - Mar 27 2009

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1167525

Cite this

Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. / Hübner, Wolfgang; McNerney, Gregory P.; Chen, Ping; Dale, Benjamin M.; Gordon, Ronald E.; Chuang, Frank; Li, Xiao Dong; Asmuth, David; Huser, Thomas R; Chen, Benjamin K.

In: Science, Vol. 323, No. 5922, 27.03.2009, p. 1743-1747.

Research output: Contribution to journal › Article › peer-review

@article{58f950e5d95b41929c2584da4ba8b3fb,

title = "Quantitative 3D video microscopy of HIV transfer across T cell virological synapses",

abstract = "The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized {"}buttons{"} containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.",

author = "Wolfgang H{\"u}bner and McNerney, {Gregory P.} and Ping Chen and Dale, {Benjamin M.} and Gordon, {Ronald E.} and Frank Chuang and Li, {Xiao Dong} and David Asmuth and Huser, {Thomas R} and Chen, {Benjamin K.}",

year = "2009",

month = mar,

day = "27",

doi = "10.1126/science.1167525",

language = "English (US)",

volume = "323",

pages = "1743--1747",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5922",

}

TY - JOUR

T1 - Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

AU - Hübner, Wolfgang

AU - McNerney, Gregory P.

AU - Chen, Ping

AU - Dale, Benjamin M.

AU - Gordon, Ronald E.

AU - Chuang, Frank

AU - Li, Xiao Dong

AU - Asmuth, David

AU - Huser, Thomas R

AU - Chen, Benjamin K.

PY - 2009/3/27

Y1 - 2009/3/27

N2 - The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

AB - The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

UR - http://www.scopus.com/inward/record.url?scp=63449126562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63449126562&partnerID=8YFLogxK

U2 - 10.1126/science.1167525

DO - 10.1126/science.1167525

M3 - Article

C2 - 19325119

AN - SCOPUS:63449126562

VL - 323

SP - 1743

EP - 1747

JO - Science

JF - Science

SN - 0036-8075

IS - 5922

ER -

Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this