Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Wolfgang Hübner, Gregory P. McNerney, Ping Chen, Benjamin M. Dale, Ronald E. Gordon, Frank Chuang, Xiao Dong Li, David Asmuth, Thomas R Huser, Benjamin K. Chen

Research output: Contribution to journalArticlepeer-review

364 Scopus citations


The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

Original languageEnglish (US)
Pages (from-to)1743-1747
Number of pages5
Issue number5922
StatePublished - Mar 27 2009

ASJC Scopus subject areas

  • General


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