Quantitation of neonicotinoid insecticides, plus qualitative screening for other xenobiotics, in small-mass avian tissue samples using UHPLC high-resolution mass spectrometry

Michael S. Filigenzi, Emily E. Graves, Lisa A Tell, Karen A. Jelks, Robert H Poppenga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We developed and validated a liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analytical method for quantitatively measuring pesticide concentrations in small-body avian tissue samples using homogenized 1–2-d-old chicken carcasses as the test matrix. We quantified the following key insecticides: sulfoxaflor (sulfoximine class) and the neonicotinoids dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, and imidacloprid. We used fortified chick carcass samples to validate method accuracy (80–125% recoveries), precision (<20% relative standard deviation), and sensitivity (≤1.2 ppb) for these targeted analytes. This method also uses full-scan, high-resolution MS to screen for the presence of a wide variety of other xenobiotics in bird carcasses. The utility of our screening process was demonstrated by the detection of carbaryl in some samples. This sensitive LC-HRMS analytical method for insecticide detection in a matrix of homogenized carcass is ideal for evaluating small birds for insecticide exposure. This novel whole-carcass method may allow for research studies of small-bodied, free-ranging avian species, and could provide insight regarding their exposure to multiple classes of environmental contaminants.

Original languageEnglish (US)
JournalJournal of Veterinary Diagnostic Investigation
DOIs
StatePublished - Jan 1 2019

Fingerprint

neonicotinoid insecticides
Xenobiotics
xenobiotics
Insecticides
Mass Spectrometry
insecticides
mass spectrometry
screening
analytical methods
nitenpyram
dinotefuran
thiacloprid
clothianidin
acetamiprid
chicken carcasses
thiamethoxam
liquids
carbaryl
birds
imidacloprid

Keywords

  • Electrospray mass spectrometry
  • hummingbirds
  • insecticides
  • pesticide residues
  • pollinators
  • small-bodied birds

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Quantitation of neonicotinoid insecticides, plus qualitative screening for other xenobiotics, in small-mass avian tissue samples using UHPLC high-resolution mass spectrometry",
abstract = "We developed and validated a liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analytical method for quantitatively measuring pesticide concentrations in small-body avian tissue samples using homogenized 1–2-d-old chicken carcasses as the test matrix. We quantified the following key insecticides: sulfoxaflor (sulfoximine class) and the neonicotinoids dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, and imidacloprid. We used fortified chick carcass samples to validate method accuracy (80–125{\%} recoveries), precision (<20{\%} relative standard deviation), and sensitivity (≤1.2 ppb) for these targeted analytes. This method also uses full-scan, high-resolution MS to screen for the presence of a wide variety of other xenobiotics in bird carcasses. The utility of our screening process was demonstrated by the detection of carbaryl in some samples. This sensitive LC-HRMS analytical method for insecticide detection in a matrix of homogenized carcass is ideal for evaluating small birds for insecticide exposure. This novel whole-carcass method may allow for research studies of small-bodied, free-ranging avian species, and could provide insight regarding their exposure to multiple classes of environmental contaminants.",
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author = "Filigenzi, {Michael S.} and Graves, {Emily E.} and Tell, {Lisa A} and Jelks, {Karen A.} and Poppenga, {Robert H}",
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AU - Filigenzi, Michael S.

AU - Graves, Emily E.

AU - Tell, Lisa A

AU - Jelks, Karen A.

AU - Poppenga, Robert H

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AB - We developed and validated a liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analytical method for quantitatively measuring pesticide concentrations in small-body avian tissue samples using homogenized 1–2-d-old chicken carcasses as the test matrix. We quantified the following key insecticides: sulfoxaflor (sulfoximine class) and the neonicotinoids dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, and imidacloprid. We used fortified chick carcass samples to validate method accuracy (80–125% recoveries), precision (<20% relative standard deviation), and sensitivity (≤1.2 ppb) for these targeted analytes. This method also uses full-scan, high-resolution MS to screen for the presence of a wide variety of other xenobiotics in bird carcasses. The utility of our screening process was demonstrated by the detection of carbaryl in some samples. This sensitive LC-HRMS analytical method for insecticide detection in a matrix of homogenized carcass is ideal for evaluating small birds for insecticide exposure. This novel whole-carcass method may allow for research studies of small-bodied, free-ranging avian species, and could provide insight regarding their exposure to multiple classes of environmental contaminants.

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