Quantitation of mouse mammary tumor virus (MTV) virions by radioimmunoassay

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Abstract

A quantitative radioimmune assay for the mouse mammary tumor virus (MTV) has been developed. The assay used highly purified MTV virions which were enzymatically externally labeled with 125I. The radioimmune precipitation (RIP) of 125I MTV with anti MTV was specifically inhibited with unlabeled MTV. The degree of inhibition of RIP was directly related to the quantity of unlabeled MTV added. The assay reproducibly detected as little as 5 ng of intact virus. The RIP of 125I MTV with anti MTV was not inhibited by murine mammary cell extracts, milk, or 'C type' virus particles. Important variables in this system are discussed.

Original languageEnglish (US)
Pages (from-to)1722-1729
Number of pages8
JournalJournal of Immunology
Volume111
Issue number6
StatePublished - Dec 1 1973

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Mouse mammary tumor virus
Oncogenic Viruses
Virion
Radioimmunoassay
Breast Neoplasms
Cell Extracts
Milk
Breast
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Quantitation of mouse mammary tumor virus (MTV) virions by radioimmunoassay. / Cardiff, Robert.

In: Journal of Immunology, Vol. 111, No. 6, 01.12.1973, p. 1722-1729.

Research output: Contribution to journalArticle

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