Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences

Guadalupe Ayala, Lilia Chihu, Guillermo Perales, Genny Fierros-Zárate, Lori M. Hansen, Jay V Solnick, Joaquín Sánchez

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalMicrobial Pathogenesis
Volume37
Issue number3
DOIs
StatePublished - Sep 2004

Keywords

  • Mexican Helicobacter pylori
  • mRNA quantitation
  • Multi-modal gene regulation
  • Promoter differences
  • RT-PCR

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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