Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences

Guadalupe Ayala, Lilia Chihu, Guillermo Perales, Genny Fierros-Zárate, Lori M. Hansen, Jay V Solnick, Joaquín Sánchez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalMicrobial Pathogenesis
Volume37
Issue number3
DOIs
StatePublished - Sep 2004

Fingerprint

Gene Fusion
Pylorus
Cytotoxins
Reporter Genes
Helicobacter pylori
Real-Time Polymerase Chain Reaction
Messenger RNA
In Vitro Techniques

Keywords

  • Mexican Helicobacter pylori
  • mRNA quantitation
  • Multi-modal gene regulation
  • Promoter differences
  • RT-PCR

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences. / Ayala, Guadalupe; Chihu, Lilia; Perales, Guillermo; Fierros-Zárate, Genny; Hansen, Lori M.; Solnick, Jay V; Sánchez, Joaquín.

In: Microbial Pathogenesis, Vol. 37, No. 3, 09.2004, p. 163-167.

Research output: Contribution to journalArticle

Ayala, Guadalupe ; Chihu, Lilia ; Perales, Guillermo ; Fierros-Zárate, Genny ; Hansen, Lori M. ; Solnick, Jay V ; Sánchez, Joaquín. / Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences. In: Microbial Pathogenesis. 2004 ; Vol. 37, No. 3. pp. 163-167.
@article{f0412a6819b54a5b9651a1e0ebab6bea,
title = "Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences",
abstract = "Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.",
keywords = "Mexican Helicobacter pylori, mRNA quantitation, Multi-modal gene regulation, Promoter differences, RT-PCR",
author = "Guadalupe Ayala and Lilia Chihu and Guillermo Perales and Genny Fierros-Z{\'a}rate and Hansen, {Lori M.} and Solnick, {Jay V} and Joaqu{\'i}n S{\'a}nchez",
year = "2004",
month = "9",
doi = "10.1016/j.micpath.2004.06.003",
language = "English (US)",
volume = "37",
pages = "163--167",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences

AU - Ayala, Guadalupe

AU - Chihu, Lilia

AU - Perales, Guillermo

AU - Fierros-Zárate, Genny

AU - Hansen, Lori M.

AU - Solnick, Jay V

AU - Sánchez, Joaquín

PY - 2004/9

Y1 - 2004/9

N2 - Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.

AB - Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.

KW - Mexican Helicobacter pylori

KW - mRNA quantitation

KW - Multi-modal gene regulation

KW - Promoter differences

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=4444307920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444307920&partnerID=8YFLogxK

U2 - 10.1016/j.micpath.2004.06.003

DO - 10.1016/j.micpath.2004.06.003

M3 - Article

VL - 37

SP - 163

EP - 167

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 3

ER -