Abstract
Using fluorescence correlation spectroscopy, we measured a dissociation constant of 20 nM between EGFP-labeled LcrV from Yersinia pestis and its cognate membrane-bound protein YopB inserted into a lipid nanodisc. The combination of fluorescence correlation spectroscopy and nanodisc technologies provides a powerful approach to accurately measure binding constants of interactions between membrane bound and soluble proteins in solution. Straightforward sample preparation, acquisition, and analysis procedures make this combined technology attractive for accurately measuring binding kinetics for this important class of protein-protein interactions.
Original language | English (US) |
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Journal | Biophysical Journal |
Volume | 106 |
Issue number | 2 |
DOIs | |
State | Published - Jan 21 2014 |
ASJC Scopus subject areas
- Biophysics