Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy

A. Daniel McCartt; Ted J. Ognibene; Graham Bench; Ken W Turteltaub

doi:10.1021/acs.analchem.6b02054

Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy

A. Daniel McCartt, Ted J. Ognibene, Graham Bench, Ken W Turteltaub

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed Guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.

Original language	English (US)
Pages (from-to)	8714-8719
Number of pages	6
Journal	Analytical Chemistry
Volume	88
Issue number	17
DOIs	https://doi.org/10.1021/acs.analchem.6b02054
State	Published - Sep 6 2016
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/acs.analchem.6b02054

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title = "Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy",

abstract = "A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed Guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.",

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AU - McCartt, A. Daniel

AU - Ognibene, Ted J.

AU - Bench, Graham

AU - Turteltaub, Ken W

PY - 2016/9/6

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AB - A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed Guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.

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Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy

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