Abstract
A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed Guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.
Original language | English (US) |
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Pages (from-to) | 8714-8719 |
Number of pages | 6 |
Journal | Analytical Chemistry |
Volume | 88 |
Issue number | 17 |
DOIs | |
State | Published - Sep 6 2016 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry