Abstract
Oligodendrocytes in neonatal rat forebrain cultures grow either in isolation of other cells or upon astrocytes. Populations of both types of oligodendrocytes were used to quantify the effects of astrocytes on oligodendroglial morphology. Changes in oligodendroglial size and shape were determined by measurement of total process length, cell area, growth area, and fractal dimension. The directionality of process growth, quantified by measurement of the axes of growth, was also compared. Isolated oligodendrocytes exhibited greater total process length, greater cellular area, larger growth area, and a more complex boundary than oligodendrocytes growing upon astrocytes. Analysis of the axes of cellular growth revealed that the processes of isolated oligodendrocytes exhibited radial symmetry, whereas the processes of oligodendrocytes growing upon astrocytes were limited to an area demarcated by the astrocytic processes. These data suggest that, in neuron-free culture, the growth of oligodendroglial processes is modified by underlying astrocytic processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 219-228 |
| Number of pages | 10 |
| Journal | Journal of Neuroscience Research |
| Volume | 49 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 15 1997 |
| Externally published | Yes |
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Keywords
- Astrocytes
- Morphology
- Oligodendrocytes
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Quantification of the effects of astrocytes on oligodendroglial morphology. / Morley, M.; Pleasure, David E; Kreider, B.
In: Journal of Neuroscience Research, Vol. 49, No. 2, 15.07.1997, p. 219-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantification of the effects of astrocytes on oligodendroglial morphology
AU - Morley, M.
AU - Pleasure, David E
AU - Kreider, B.
PY - 1997/7/15
Y1 - 1997/7/15
N2 - Oligodendrocytes in neonatal rat forebrain cultures grow either in isolation of other cells or upon astrocytes. Populations of both types of oligodendrocytes were used to quantify the effects of astrocytes on oligodendroglial morphology. Changes in oligodendroglial size and shape were determined by measurement of total process length, cell area, growth area, and fractal dimension. The directionality of process growth, quantified by measurement of the axes of growth, was also compared. Isolated oligodendrocytes exhibited greater total process length, greater cellular area, larger growth area, and a more complex boundary than oligodendrocytes growing upon astrocytes. Analysis of the axes of cellular growth revealed that the processes of isolated oligodendrocytes exhibited radial symmetry, whereas the processes of oligodendrocytes growing upon astrocytes were limited to an area demarcated by the astrocytic processes. These data suggest that, in neuron-free culture, the growth of oligodendroglial processes is modified by underlying astrocytic processes.
AB - Oligodendrocytes in neonatal rat forebrain cultures grow either in isolation of other cells or upon astrocytes. Populations of both types of oligodendrocytes were used to quantify the effects of astrocytes on oligodendroglial morphology. Changes in oligodendroglial size and shape were determined by measurement of total process length, cell area, growth area, and fractal dimension. The directionality of process growth, quantified by measurement of the axes of growth, was also compared. Isolated oligodendrocytes exhibited greater total process length, greater cellular area, larger growth area, and a more complex boundary than oligodendrocytes growing upon astrocytes. Analysis of the axes of cellular growth revealed that the processes of isolated oligodendrocytes exhibited radial symmetry, whereas the processes of oligodendrocytes growing upon astrocytes were limited to an area demarcated by the astrocytic processes. These data suggest that, in neuron-free culture, the growth of oligodendroglial processes is modified by underlying astrocytic processes.
KW - Astrocytes
KW - Morphology
KW - Oligodendrocytes
UR - http://www.scopus.com/inward/record.url?scp=0030808943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030808943&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4547(19970715)49:2<219::AID-JNR10>3.0.CO;2-W
DO - 10.1002/(SICI)1097-4547(19970715)49:2<219::AID-JNR10>3.0.CO;2-W
M3 - Article
C2 - 9272644
AN - SCOPUS:0030808943
VL - 49
SP - 219
EP - 228
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 2
ER -