Quantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.

Original languageEnglish (US)
Pages (from-to)4930-4944
Number of pages15
JournalOptics Express
Volume16
Issue number7
DOIs
StatePublished - Mar 31 2008

Fingerprint

ischemia
exponential functions
kidneys
progressions
organs
time constant
rats
excitation
probes
products
profiles

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Quantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation. / Raman, Rajesh N.; Pivetti, Christopher D.; Matthews, Dennis L; Troppmann, Christoph; Demos, Stavros G.

In: Optics Express, Vol. 16, No. 7, 31.03.2008, p. 4930-4944.

Research output: Contribution to journalArticle

@article{cade916dbb024a179b7d7c293cc68f0e,
title = "Quantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation",
abstract = "We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.",
author = "Raman, {Rajesh N.} and Pivetti, {Christopher D.} and Matthews, {Dennis L} and Christoph Troppmann and Demos, {Stavros G.}",
year = "2008",
month = "3",
day = "31",
doi = "10.1364/OE.16.004930",
language = "English (US)",
volume = "16",
pages = "4930--4944",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "7",

}

TY - JOUR

T1 - Quantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation

AU - Raman, Rajesh N.

AU - Pivetti, Christopher D.

AU - Matthews, Dennis L

AU - Troppmann, Christoph

AU - Demos, Stavros G.

PY - 2008/3/31

Y1 - 2008/3/31

N2 - We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.

AB - We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.

UR - http://www.scopus.com/inward/record.url?scp=41649099058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41649099058&partnerID=8YFLogxK

U2 - 10.1364/OE.16.004930

DO - 10.1364/OE.16.004930

M3 - Article

C2 - 18542592

AN - SCOPUS:41649099058

VL - 16

SP - 4930

EP - 4944

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 7

ER -