Quantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.

Original languageEnglish (US)
Pages (from-to)4930-4944
Number of pages15
JournalOptics Express
Volume16
Issue number7
DOIs
StatePublished - Mar 31 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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