Abstract
Objective: To characterize HIV-1 specific cellular immune responses at mucosal surfaces using a rapid, sensitive enzyme-linked immuno-spot (ELISPOT) technique. Design: Cervicovaginal mononuclear cells obtained from cytobrush and cervicovaginal lavage were assessed for production of interferon-gamma (IFN-γ) in response to stimulation by HIV-1 antigens. HIV-1 specific responses were compared in a cross-sectional study of two HIV-1-positive patient groups: women not currently on anti-retroviral therapy with peripheral CD4 cell counts > 250 x 106/l (n = 12); and women on highly active antiretroviral therapy (HAART) (n = 9). Methods: Mononuclear cells from peripheral blood or cervicovaginal specimens were assessed in an ELISPOT assay for responses to HIV-1 antigens expressed by recombinant vaccinia viruses. This assay detects primarily CD8 T cells and shows good correlation with MHC class I tetramer staining of cytotoxic T lymphocytes. Results: HIV-1 specific IFN-γ spot-forming cells were detected in cervicovaginal samples of one out of nine women (11%) on HAART and five out of 12 women (42%) not currently on HAART. In peripheral blood mononuclear cells, HIV-1 specific IFN-γ spot-forming cells were significantly more numerous in women not currently on HAART than in women on HAART (P = 0.009). In most cases, antigens recognized by mucosal T cells were also recognized by PBMC; however, there were exceptions. Conclusions: HIV-1-specific antigen-reactive T cells may be detected in routine, non-invasive gynecological specimens. The results suggest that cervicovaginal HIV-1-specific T cells may be less numerous in individuals on HAART than in those not on HAART, as shown previously for HIV-1-specific cytotoxic T lymphocytes in the peripheral blood. (C) 2000 Lippincott Williams and Wilkins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1911-1915 |
| Number of pages | 5 |
| Journal | AIDS |
| Volume | 14 |
| Issue number | 13 |
| DOIs | |
| State | Published - Sep 8 2000 |
| Externally published | Yes |
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Keywords
- Antiretroviral therapy
- Cellular immunity
- Gynecology
- HAART
- Obstetrics
- Women
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. / Shacklett, Barbara; Cu-Uvin, Susan; Beadle, Thomas J.; Pace, Christine A.; Fast, Noam M.; Donahue, Shannon M.; Caliendo, Angela M.; Flanigan, Timothy P.; Carpenter, Charles C J; Nixon, Douglas F.
In: AIDS, Vol. 14, No. 13, 08.09.2000, p. 1911-1915.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface
AU - Shacklett, Barbara
AU - Cu-Uvin, Susan
AU - Beadle, Thomas J.
AU - Pace, Christine A.
AU - Fast, Noam M.
AU - Donahue, Shannon M.
AU - Caliendo, Angela M.
AU - Flanigan, Timothy P.
AU - Carpenter, Charles C J
AU - Nixon, Douglas F.
PY - 2000/9/8
Y1 - 2000/9/8
N2 - Objective: To characterize HIV-1 specific cellular immune responses at mucosal surfaces using a rapid, sensitive enzyme-linked immuno-spot (ELISPOT) technique. Design: Cervicovaginal mononuclear cells obtained from cytobrush and cervicovaginal lavage were assessed for production of interferon-gamma (IFN-γ) in response to stimulation by HIV-1 antigens. HIV-1 specific responses were compared in a cross-sectional study of two HIV-1-positive patient groups: women not currently on anti-retroviral therapy with peripheral CD4 cell counts > 250 x 106/l (n = 12); and women on highly active antiretroviral therapy (HAART) (n = 9). Methods: Mononuclear cells from peripheral blood or cervicovaginal specimens were assessed in an ELISPOT assay for responses to HIV-1 antigens expressed by recombinant vaccinia viruses. This assay detects primarily CD8 T cells and shows good correlation with MHC class I tetramer staining of cytotoxic T lymphocytes. Results: HIV-1 specific IFN-γ spot-forming cells were detected in cervicovaginal samples of one out of nine women (11%) on HAART and five out of 12 women (42%) not currently on HAART. In peripheral blood mononuclear cells, HIV-1 specific IFN-γ spot-forming cells were significantly more numerous in women not currently on HAART than in women on HAART (P = 0.009). In most cases, antigens recognized by mucosal T cells were also recognized by PBMC; however, there were exceptions. Conclusions: HIV-1-specific antigen-reactive T cells may be detected in routine, non-invasive gynecological specimens. The results suggest that cervicovaginal HIV-1-specific T cells may be less numerous in individuals on HAART than in those not on HAART, as shown previously for HIV-1-specific cytotoxic T lymphocytes in the peripheral blood. (C) 2000 Lippincott Williams and Wilkins.
AB - Objective: To characterize HIV-1 specific cellular immune responses at mucosal surfaces using a rapid, sensitive enzyme-linked immuno-spot (ELISPOT) technique. Design: Cervicovaginal mononuclear cells obtained from cytobrush and cervicovaginal lavage were assessed for production of interferon-gamma (IFN-γ) in response to stimulation by HIV-1 antigens. HIV-1 specific responses were compared in a cross-sectional study of two HIV-1-positive patient groups: women not currently on anti-retroviral therapy with peripheral CD4 cell counts > 250 x 106/l (n = 12); and women on highly active antiretroviral therapy (HAART) (n = 9). Methods: Mononuclear cells from peripheral blood or cervicovaginal specimens were assessed in an ELISPOT assay for responses to HIV-1 antigens expressed by recombinant vaccinia viruses. This assay detects primarily CD8 T cells and shows good correlation with MHC class I tetramer staining of cytotoxic T lymphocytes. Results: HIV-1 specific IFN-γ spot-forming cells were detected in cervicovaginal samples of one out of nine women (11%) on HAART and five out of 12 women (42%) not currently on HAART. In peripheral blood mononuclear cells, HIV-1 specific IFN-γ spot-forming cells were significantly more numerous in women not currently on HAART than in women on HAART (P = 0.009). In most cases, antigens recognized by mucosal T cells were also recognized by PBMC; however, there were exceptions. Conclusions: HIV-1-specific antigen-reactive T cells may be detected in routine, non-invasive gynecological specimens. The results suggest that cervicovaginal HIV-1-specific T cells may be less numerous in individuals on HAART than in those not on HAART, as shown previously for HIV-1-specific cytotoxic T lymphocytes in the peripheral blood. (C) 2000 Lippincott Williams and Wilkins.
KW - Antiretroviral therapy
KW - Cellular immunity
KW - Gynecology
KW - HAART
KW - Obstetrics
KW - Women
UR - http://www.scopus.com/inward/record.url?scp=0034622954&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034622954&partnerID=8YFLogxK
U2 - 10.1097/00002030-200009080-00005
DO - 10.1097/00002030-200009080-00005
M3 - Article
C2 - 10997394
AN - SCOPUS:0034622954
VL - 14
SP - 1911
EP - 1915
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 13
ER -