Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface

Barbara Shacklett, Susan Cu-Uvin, Thomas J. Beadle, Christine A. Pace, Noam M. Fast, Shannon M. Donahue, Angela M. Caliendo, Timothy P. Flanigan, Charles C J Carpenter, Douglas F. Nixon

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objective: To characterize HIV-1 specific cellular immune responses at mucosal surfaces using a rapid, sensitive enzyme-linked immuno-spot (ELISPOT) technique. Design: Cervicovaginal mononuclear cells obtained from cytobrush and cervicovaginal lavage were assessed for production of interferon-gamma (IFN-γ) in response to stimulation by HIV-1 antigens. HIV-1 specific responses were compared in a cross-sectional study of two HIV-1-positive patient groups: women not currently on anti-retroviral therapy with peripheral CD4 cell counts > 250 x 106/l (n = 12); and women on highly active antiretroviral therapy (HAART) (n = 9). Methods: Mononuclear cells from peripheral blood or cervicovaginal specimens were assessed in an ELISPOT assay for responses to HIV-1 antigens expressed by recombinant vaccinia viruses. This assay detects primarily CD8 T cells and shows good correlation with MHC class I tetramer staining of cytotoxic T lymphocytes. Results: HIV-1 specific IFN-γ spot-forming cells were detected in cervicovaginal samples of one out of nine women (11%) on HAART and five out of 12 women (42%) not currently on HAART. In peripheral blood mononuclear cells, HIV-1 specific IFN-γ spot-forming cells were significantly more numerous in women not currently on HAART than in women on HAART (P = 0.009). In most cases, antigens recognized by mucosal T cells were also recognized by PBMC; however, there were exceptions. Conclusions: HIV-1-specific antigen-reactive T cells may be detected in routine, non-invasive gynecological specimens. The results suggest that cervicovaginal HIV-1-specific T cells may be less numerous in individuals on HAART than in those not on HAART, as shown previously for HIV-1-specific cytotoxic T lymphocytes in the peripheral blood. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)1911-1915
Number of pages5
JournalAIDS
Volume14
Issue number13
DOIs
StatePublished - Sep 8 2000
Externally publishedYes

Fingerprint

HIV-1
Highly Active Antiretroviral Therapy
T-Lymphocytes
HIV Antigens
Interferon-gamma
Cytotoxic T-Lymphocytes
Blood Cells
Antigens
Vaccinia virus
Therapeutic Irrigation
Enzymes
CD4 Lymphocyte Count
Cellular Immunity
Cross-Sectional Studies
Staining and Labeling

Keywords

  • Antiretroviral therapy
  • Cellular immunity
  • Gynecology
  • HAART
  • Obstetrics
  • Women

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Shacklett, B., Cu-Uvin, S., Beadle, T. J., Pace, C. A., Fast, N. M., Donahue, S. M., ... Nixon, D. F. (2000). Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. AIDS, 14(13), 1911-1915. https://doi.org/10.1097/00002030-200009080-00005

Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. / Shacklett, Barbara; Cu-Uvin, Susan; Beadle, Thomas J.; Pace, Christine A.; Fast, Noam M.; Donahue, Shannon M.; Caliendo, Angela M.; Flanigan, Timothy P.; Carpenter, Charles C J; Nixon, Douglas F.

In: AIDS, Vol. 14, No. 13, 08.09.2000, p. 1911-1915.

Research output: Contribution to journalArticle

Shacklett, B, Cu-Uvin, S, Beadle, TJ, Pace, CA, Fast, NM, Donahue, SM, Caliendo, AM, Flanigan, TP, Carpenter, CCJ & Nixon, DF 2000, 'Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface', AIDS, vol. 14, no. 13, pp. 1911-1915. https://doi.org/10.1097/00002030-200009080-00005
Shacklett, Barbara ; Cu-Uvin, Susan ; Beadle, Thomas J. ; Pace, Christine A. ; Fast, Noam M. ; Donahue, Shannon M. ; Caliendo, Angela M. ; Flanigan, Timothy P. ; Carpenter, Charles C J ; Nixon, Douglas F. / Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. In: AIDS. 2000 ; Vol. 14, No. 13. pp. 1911-1915.
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AU - Pace, Christine A.

AU - Fast, Noam M.

AU - Donahue, Shannon M.

AU - Caliendo, Angela M.

AU - Flanigan, Timothy P.

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N2 - Objective: To characterize HIV-1 specific cellular immune responses at mucosal surfaces using a rapid, sensitive enzyme-linked immuno-spot (ELISPOT) technique. Design: Cervicovaginal mononuclear cells obtained from cytobrush and cervicovaginal lavage were assessed for production of interferon-gamma (IFN-γ) in response to stimulation by HIV-1 antigens. HIV-1 specific responses were compared in a cross-sectional study of two HIV-1-positive patient groups: women not currently on anti-retroviral therapy with peripheral CD4 cell counts > 250 x 106/l (n = 12); and women on highly active antiretroviral therapy (HAART) (n = 9). Methods: Mononuclear cells from peripheral blood or cervicovaginal specimens were assessed in an ELISPOT assay for responses to HIV-1 antigens expressed by recombinant vaccinia viruses. This assay detects primarily CD8 T cells and shows good correlation with MHC class I tetramer staining of cytotoxic T lymphocytes. Results: HIV-1 specific IFN-γ spot-forming cells were detected in cervicovaginal samples of one out of nine women (11%) on HAART and five out of 12 women (42%) not currently on HAART. In peripheral blood mononuclear cells, HIV-1 specific IFN-γ spot-forming cells were significantly more numerous in women not currently on HAART than in women on HAART (P = 0.009). In most cases, antigens recognized by mucosal T cells were also recognized by PBMC; however, there were exceptions. Conclusions: HIV-1-specific antigen-reactive T cells may be detected in routine, non-invasive gynecological specimens. The results suggest that cervicovaginal HIV-1-specific T cells may be less numerous in individuals on HAART than in those not on HAART, as shown previously for HIV-1-specific cytotoxic T lymphocytes in the peripheral blood. (C) 2000 Lippincott Williams and Wilkins.

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