Quantification of hepatic steatosis with a multistep adaptive fitting MRI approach: Prospective validation against MR spectroscopy

Mustafa R. Bashir, Xiaodong Zhong, Marcel D. Nickel, Ghaneh Fananapazir, Stephan A R Kannengiesser, Berthold Kiefer, Brian M. Dale

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


OBJECTIVE. The purpose of this study is to prospectively compare hybrid and complex chemical shift-based MRI fat quantification methods against MR spectroscopy (MRS) lor the measurement of hepatic steatosis. SUBJECTS AND METHODS. Forty-two subjects (18 men and 24 women; mean ± SD age, 52.8 ± 14 years) were prospectively enrolled and imaged at 3 T with a chemical shift-based MRI sequence and a single-voxel MRS sequence, each in one breath-hold, Proton density fat fraction and rate constant (R<inf>2</inf>∗) using both single-and dual-R<inf>2</inf>∗ hybrid lilting methods, as well as proton density fat fraction and R<inf>2</inf>∗ maps using a complex lilting method, were generated. A single radiologist colocalized volumes of interest on the proton density fat fraction and R<inf>2</inf>∗ maps according to the spectroscopy measurement voxel. Agreement among the three MRI methods and the MRS proton density fat fraction values was assessed using linear regression, intraclass correlation coefficient (ICC), and Bland-Altman analysis. RESULTS. Correlation between the MRI and MRS measures of proton density fat fraction was excellent. Linear regression coefficients ranged from 0,98 to 1.01, and intercepts ranged from-1,12% to 0.49%, Agreement measured by ICC was also excellent (0.99 for all three methods). Bland-Altman analysis showed excellent agreement, with mean differences of-1.0% to 0.6% (SD, 1.3-1.6%). CONCLUSION. The described MRI-based liver proton density fat fraction measures are clinically feasible and accurate. The validation of proton density tat traction quantification methods is an important step toward wide availability and acceptance of the MRI-based measurement of proton density fat fraction as an accurate and generalizable hioinarker.

Original languageEnglish (US)
Pages (from-to)297-306
Number of pages10
JournalAmerican Journal of Roentgenology
Issue number2
StatePublished - Feb 1 2015
Externally publishedYes


  • Fat fraction
  • Hepatic steatosis
  • Proton density fat fraction

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'Quantification of hepatic steatosis with a multistep adaptive fitting MRI approach: Prospective validation against MR spectroscopy'. Together they form a unique fingerprint.

Cite this