Quantification of cardiac fiber orientation using optical coherence tomography

Christine P. Fleming, Crystal M Ripplinger, Bryan Webb, Igor R. Efimov, Andrew M. Rollins

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.

Original languageEnglish (US)
Article number030505
JournalJournal of Biomedical Optics
Issue number3
StatePublished - 2008
Externally publishedYes


  • biomedical optics
  • cardiac
  • image processing
  • optical coherence tomography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry


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