Qualitative analysis of algal secretions with multiple mass spectrometric platforms

Tobias Kind, John K. Meissen, Dawei Yang, Fernando Nocito, Arpana Vaniya, Yu Shen Cheng, Jean S. VanderGheynst, Oliver Fiehn

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Lipid secretions from algae pose a great opportunity for engineering biofueler feedstocks. The lipid exudates could be interesting from a process engineering perspective because lipids could be collected directly from the medium without harvesting and disrupting cells. We here report on the extracellular secretions of algal metabolites from the strain UTEX 2341 (. Chlorella minutissima) into the culture medium. No detailed analysis of these lipid secretions has been performed to date. Using multiple mass spectrometric platforms, we observed around 1000 compounds and were able to annotate 50 lipids by means of liquid chromatography coupled to accurate mass quadrupole time-of-flight mass spectrometry (LC-QTOF), direct infusion with positive and negative electrospray ion trap mass spectrometry and gas chromatography coupled to mass spectrometry (GC-MS). These compounds were annotated by tandem mass spectral (MS/MS) database matching and retention time range filtering. We observed a series of triacylglycerols (TG), sulfoquinovosyldiacylglycerols (SQDG), phosphatidylinositols and phosphatidylglycerols, as well as betaine lipids diacylglyceryl-N,N,N-trimethylhomoserines (DGTS).

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalJournal of Chromatography A
Volume1244
DOIs
StatePublished - Jun 29 2012

Fingerprint

Lipids
Mass spectrometry
Mass Spectrometry
Chlorella
Betaine
Phosphatidylglycerols
Liquid chromatography
Process engineering
Exudates and Transudates
Algae
Metabolites
Phosphatidylinositols
Liquid Chromatography
Gas chromatography
Gas Chromatography-Mass Spectrometry
Feedstocks
Culture Media
Triglycerides
Databases
Ions

Keywords

  • Algae
  • Biofuel
  • GC-MS
  • LC-MS
  • Lipids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Kind, T., Meissen, J. K., Yang, D., Nocito, F., Vaniya, A., Cheng, Y. S., ... Fiehn, O. (2012). Qualitative analysis of algal secretions with multiple mass spectrometric platforms. Journal of Chromatography A, 1244, 139-147. https://doi.org/10.1016/j.chroma.2012.04.074

Qualitative analysis of algal secretions with multiple mass spectrometric platforms. / Kind, Tobias; Meissen, John K.; Yang, Dawei; Nocito, Fernando; Vaniya, Arpana; Cheng, Yu Shen; VanderGheynst, Jean S.; Fiehn, Oliver.

In: Journal of Chromatography A, Vol. 1244, 29.06.2012, p. 139-147.

Research output: Contribution to journalArticle

Kind, T, Meissen, JK, Yang, D, Nocito, F, Vaniya, A, Cheng, YS, VanderGheynst, JS & Fiehn, O 2012, 'Qualitative analysis of algal secretions with multiple mass spectrometric platforms', Journal of Chromatography A, vol. 1244, pp. 139-147. https://doi.org/10.1016/j.chroma.2012.04.074
Kind, Tobias ; Meissen, John K. ; Yang, Dawei ; Nocito, Fernando ; Vaniya, Arpana ; Cheng, Yu Shen ; VanderGheynst, Jean S. ; Fiehn, Oliver. / Qualitative analysis of algal secretions with multiple mass spectrometric platforms. In: Journal of Chromatography A. 2012 ; Vol. 1244. pp. 139-147.
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