QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits

Fang Z. Zhang, Carol Wagstaff, Anne M. Rae, Arinder K. Sihota, C. William Keevil, Steve D. Rothwell, Graham J J Clarkson, Richard W Michelmore, María José Truco, Mark S. Dixon, Gail Taylor

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Developmental and biophysical leaf characteristics that influence post-harvest shelf life in lettuce, an important leafy crop, have been examined. The traits were studied using 60 informative F9 recombinant inbed lines (RILs) derived from a cross between cultivated lettuce (Lactuca sativa cv. Salinas) and wild lettuce (L. serriola acc. UC96US23). Quantitative trait loci (QTLs) for shelf life co-located most closely with those for leaf biophysical properties such as plasticity, elasticity, and breakstrength, suggesting that these are appropriate targets for molecular breeding for improved shelf life. Significant correlations were found between shelf life and leaf size, leaf weight, leaf chlorophyll content, leaf stomatal index, and epidermal cell number per leaf, indicating that these pre-harvest leaf development traits confer post-harvest properties. By studying the population in two contrasting environments in northern and southern Europe, the genotype by environment interaction effects of the QTLs relevant to leaf development and shelf life were assessed. In total, 107 QTLs, distributed on all nine linkage groups, were detected from the 29 traits. Only five QTLs were common in both environments. Several areas where many QTLs co-located (hotspots) on the genome were identified, with relatively little overlap between developmental hotspots and those relating to shelf life. However, QTLs for leaf biophysical properties (breakstrength, plasticity, and elasticity) and cell area correlated well with shelf life, confirming that the ideal ideotype lettuce should have small cells with strong cell walls. The identification of QTLs for leaf development, strength, and longevity will lead to a better understanding of processability at a genetic and cellular level, and allow the improvement of salad leaf quality through marker-assisted breeding.

Original languageEnglish (US)
Pages (from-to)1433-1449
Number of pages17
JournalJournal of Experimental Botany
Volume58
Issue number6
DOIs
StatePublished - May 2007

Fingerprint

Lettuce
Quantitative Trait Loci
lettuce
quantitative trait loci
shelf life
leaves
leaf development
Elasticity
elasticity (mechanics)
DNA Shuffling
Lactuca serriola
ideotypes
Chlorophyll
Cell Wall
salads
Breeding
Southern European region
breeding
Northern European region
Lactuca sativa

Keywords

  • Biomechanical properties
  • Biophysical
  • Leaf development
  • Lettuce
  • Microbiology
  • Post-harvest
  • QTLs
  • Shelf life

ASJC Scopus subject areas

  • Plant Science

Cite this

Zhang, F. Z., Wagstaff, C., Rae, A. M., Sihota, A. K., Keevil, C. W., Rothwell, S. D., ... Taylor, G. (2007). QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits. Journal of Experimental Botany, 58(6), 1433-1449. https://doi.org/10.1093/jxb/erm006

QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits. / Zhang, Fang Z.; Wagstaff, Carol; Rae, Anne M.; Sihota, Arinder K.; Keevil, C. William; Rothwell, Steve D.; Clarkson, Graham J J; Michelmore, Richard W; Truco, María José; Dixon, Mark S.; Taylor, Gail.

In: Journal of Experimental Botany, Vol. 58, No. 6, 05.2007, p. 1433-1449.

Research output: Contribution to journalArticle

Zhang, FZ, Wagstaff, C, Rae, AM, Sihota, AK, Keevil, CW, Rothwell, SD, Clarkson, GJJ, Michelmore, RW, Truco, MJ, Dixon, MS & Taylor, G 2007, 'QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits', Journal of Experimental Botany, vol. 58, no. 6, pp. 1433-1449. https://doi.org/10.1093/jxb/erm006
Zhang, Fang Z. ; Wagstaff, Carol ; Rae, Anne M. ; Sihota, Arinder K. ; Keevil, C. William ; Rothwell, Steve D. ; Clarkson, Graham J J ; Michelmore, Richard W ; Truco, María José ; Dixon, Mark S. ; Taylor, Gail. / QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits. In: Journal of Experimental Botany. 2007 ; Vol. 58, No. 6. pp. 1433-1449.
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abstract = "Developmental and biophysical leaf characteristics that influence post-harvest shelf life in lettuce, an important leafy crop, have been examined. The traits were studied using 60 informative F9 recombinant inbed lines (RILs) derived from a cross between cultivated lettuce (Lactuca sativa cv. Salinas) and wild lettuce (L. serriola acc. UC96US23). Quantitative trait loci (QTLs) for shelf life co-located most closely with those for leaf biophysical properties such as plasticity, elasticity, and breakstrength, suggesting that these are appropriate targets for molecular breeding for improved shelf life. Significant correlations were found between shelf life and leaf size, leaf weight, leaf chlorophyll content, leaf stomatal index, and epidermal cell number per leaf, indicating that these pre-harvest leaf development traits confer post-harvest properties. By studying the population in two contrasting environments in northern and southern Europe, the genotype by environment interaction effects of the QTLs relevant to leaf development and shelf life were assessed. In total, 107 QTLs, distributed on all nine linkage groups, were detected from the 29 traits. Only five QTLs were common in both environments. Several areas where many QTLs co-located (hotspots) on the genome were identified, with relatively little overlap between developmental hotspots and those relating to shelf life. However, QTLs for leaf biophysical properties (breakstrength, plasticity, and elasticity) and cell area correlated well with shelf life, confirming that the ideal ideotype lettuce should have small cells with strong cell walls. The identification of QTLs for leaf development, strength, and longevity will lead to a better understanding of processability at a genetic and cellular level, and allow the improvement of salad leaf quality through marker-assisted breeding.",
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AU - Zhang, Fang Z.

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AU - Sihota, Arinder K.

AU - Keevil, C. William

AU - Rothwell, Steve D.

AU - Clarkson, Graham J J

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AU - Dixon, Mark S.

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