Pyogranulomatous Pleuropneumonia and Mediastinitis in Ferrets (Mustela putorius furo) associated with Pseudomonas luteola Infection

J. Martínez, J. Martorell, M. L. Abarca, A. Olvera, A. Ramis, Leslie Woods, N. Cheville, C. Juan-Sallés, A. Moya, A. Riera, S. Soto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Between 2008 and 2009, three pet ferrets from different sources presented with acute episode of dyspnoea. Cytological examination of pleural exudates revealed severe purulent inflammation with abundant clusters of rod-shaped microorganisms with a clear surrounding halo. Treatment was ineffective and the ferrets died 2-5 days later. Two ferrets were subjected to necropsy examination, which revealed pyothorax, mediastinal lymphadenopathy and multiple white nodules (1-2. mm) in the lungs. Microscopical examination showed multifocal necrotizing-pyogranulomatous pleuropneumonia and lymphadenitis with aggregates of encapsulated microorganisms, some of which were positively stained by periodic acid-Schiff and alcian blue. In-situ hybridization for Pneumocystis spp., Ziehl-Neelsen staining and immunohistochemistry for distemper, coronavirus and influenza antigen were negative in all cases. Electron microscopically, the bacteria were 2-3μm long with a thick electron-lucent capsule. Microbiology from one ferret yielded a pure culture of gram-negative bacteria identified phenotypically as Pseudomonas luteola. This speciation was later confirmed by 16S RNA gene amplification.

Original languageEnglish (US)
Pages (from-to)4-10
Number of pages7
JournalJournal of Comparative Pathology
Volume146
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Pseudomonas luteola
Pleuropneumonia
Mediastinitis
Pseudomonas Infections
Ferrets
ferrets
infection
Pneumocystis
Distemper
electrons
Electrons
Pleural Empyema
lymphadenitis
distemper
microorganisms
Alcian Blue
lymphatic diseases
Lymphadenitis
Coronavirus
Periodic Acid

Keywords

  • Ferret
  • Lymphadenitis
  • Pleuropneumonia
  • Pseudomonas luteola

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • veterinary(all)

Cite this

Pyogranulomatous Pleuropneumonia and Mediastinitis in Ferrets (Mustela putorius furo) associated with Pseudomonas luteola Infection. / Martínez, J.; Martorell, J.; Abarca, M. L.; Olvera, A.; Ramis, A.; Woods, Leslie; Cheville, N.; Juan-Sallés, C.; Moya, A.; Riera, A.; Soto, S.

In: Journal of Comparative Pathology, Vol. 146, No. 1, 01.2012, p. 4-10.

Research output: Contribution to journalArticle

Martínez, J, Martorell, J, Abarca, ML, Olvera, A, Ramis, A, Woods, L, Cheville, N, Juan-Sallés, C, Moya, A, Riera, A & Soto, S 2012, 'Pyogranulomatous Pleuropneumonia and Mediastinitis in Ferrets (Mustela putorius furo) associated with Pseudomonas luteola Infection', Journal of Comparative Pathology, vol. 146, no. 1, pp. 4-10. https://doi.org/10.1016/j.jcpa.2011.03.014
Martínez, J. ; Martorell, J. ; Abarca, M. L. ; Olvera, A. ; Ramis, A. ; Woods, Leslie ; Cheville, N. ; Juan-Sallés, C. ; Moya, A. ; Riera, A. ; Soto, S. / Pyogranulomatous Pleuropneumonia and Mediastinitis in Ferrets (Mustela putorius furo) associated with Pseudomonas luteola Infection. In: Journal of Comparative Pathology. 2012 ; Vol. 146, No. 1. pp. 4-10.
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