Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy

Thomas J. Smith, R. Holland Cheng, Norman H. Olson, Peter Peterson, Elaine Chase, Richard J. Kuhn, Timothy S. Baker

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The structures of Sindbis virus and Ross River virus complexed with Fab fragments from monoclonal antibodies have been determined from cryoelectron micrographs. Both antibodies chosen for this study bind to regions of the virions that have been implicated in cell-receptor recognition and recognize epitopes on the E2 glycoprotein. The two structures show that the Fab fragments bind to the outermost tip of the trimeric envelope spike protein. Hence, the same region of both the Sindbis virus and Ross River virus envelope spike is composed of E2 and is involved in recognition of the cellular receptor.

Original languageEnglish (US)
Pages (from-to)10648-10652
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number23
DOIs
StatePublished - Nov 7 1995
Externally publishedYes

Fingerprint

Ross River virus
Sindbis Virus
Alphavirus
Cryoelectron Microscopy
Immunoglobulin Fab Fragments
Binding Sites
Virion
Epitopes
Glycoproteins
Monoclonal Antibodies
Antibodies
Proteins

Keywords

  • antibodies
  • neutralization
  • Ross River virus
  • Sindbis virus

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy. / Smith, Thomas J.; Cheng, R. Holland; Olson, Norman H.; Peterson, Peter; Chase, Elaine; Kuhn, Richard J.; Baker, Timothy S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 23, 07.11.1995, p. 10648-10652.

Research output: Contribution to journalArticle

Smith, Thomas J. ; Cheng, R. Holland ; Olson, Norman H. ; Peterson, Peter ; Chase, Elaine ; Kuhn, Richard J. ; Baker, Timothy S. / Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 23. pp. 10648-10652.
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