Purified protein delivery to activate an epigenetically silenced allele in mouse brain

Benjamin Pyles, Barbara J. Bailus, Henriette O’Geen, David Segal

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The ability to activate or repress specific genes in the brain could have a tremendous impact for understanding and treating neurological disorders. Artificial transcription factors based on zinc finger, TALE, and CRISPR/Cas9 programmable DNA-binding platforms have been widely used to regulate the expression of specific genes in cultured cells, but their delivery into the brain represents a critical challenge to apply such tools in live animals. In previous work, we developed a purified, zinc finger-based artificial transcription factor that could be injected systemically, cross the blood-brain barrier, and alter expression of a specific gene in the brain of an adult mouse model of Angelman syndrome. Importantly, our mode of delivery produced widespread distribution throughout the brain. Here we describe our most current methods for the production and purification of the factor, dosage optimization, and use of live animal fluorescence imaging to visualize the kinetics of distribution.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages227-239
Number of pages13
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1767
ISSN (Print)1064-3745

Keywords

  • Animal models
  • Engineered zinc finger protein
  • Gene therapy
  • Neurologic disease
  • Preclinical studies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Pyles, B., Bailus, B. J., O’Geen, H., & Segal, D. (2018). Purified protein delivery to activate an epigenetically silenced allele in mouse brain. In Methods in Molecular Biology (pp. 227-239). (Methods in Molecular Biology; Vol. 1767). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7774-1_12