Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

Byron E. Froman, Robert C. Tait, Clarence I. Kado, Raymond L. Rodriguez

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

Original languageEnglish (US)
Pages (from-to)331-335
Number of pages5
JournalGene
Volume28
Issue number3
DOIs
StatePublished - 1984

Keywords

  • blunt and cohesive termini
  • enzyme purification
  • phage λ
  • Recombinant DNA
  • XmaI, SmaI isoschizomers

ASJC Scopus subject areas

  • Genetics

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    Froman, B. E., Tait, R. C., Kado, C. I., & Rodriguez, R. L. (1984). Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis. Gene, 28(3), 331-335. https://doi.org/10.1016/0378-1119(84)90150-1