Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

Byron E. Froman, Robert C. Tait, Clarence I. Kado, Raymond L. Rodriguez

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

Original languageEnglish (US)
Pages (from-to)331-335
Number of pages5
Issue number3
StatePublished - 1984


  • blunt and cohesive termini
  • enzyme purification
  • phage λ
  • Recombinant DNA
  • XmaI, SmaI isoschizomers

ASJC Scopus subject areas

  • Genetics


Dive into the research topics of 'Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis'. Together they form a unique fingerprint.

Cite this