Abstract
A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 331-335 |
| Number of pages | 5 |
| Journal | Gene |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1984 |
Keywords
- blunt and cohesive termini
- enzyme purification
- phage λ
- Recombinant DNA
- XmaI, SmaI isoschizomers
ASJC Scopus subject areas
- Genetics
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Cite this
Froman, B. E., Tait, R. C., Kado, C. I., & Rodriguez, R. L. (1984). Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis. Gene, 28(3), 331-335. https://doi.org/10.1016/0378-1119(84)90150-1