Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

Byron E. Froman; Robert C. Tait; Clarence I. Kado; Raymond L. Rodriguez

doi:10.1016/0378-1119(84)90150-1

Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

Byron E. Froman, Robert C. Tait, Clarence I. Kado, Raymond L. Rodriguez

Molecular and Cellular Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

Original language	English (US)
Pages (from-to)	331-335
Number of pages	5
Journal	Gene
Volume	28
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(84)90150-1
State	Published - 1984

Keywords

blunt and cohesive termini
enzyme purification
phage λ
Recombinant DNA
XmaI, SmaI isoschizomers

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(84)90150-1

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@article{700a6fa35ed547fa87a2b32f026fbce0,

title = "Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis",

abstract = "A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.",

keywords = "blunt and cohesive termini, enzyme purification, phage λ, Recombinant DNA, XmaI, SmaI isoschizomers",

author = "Froman, {Byron E.} and Tait, {Robert C.} and Kado, {Clarence I.} and Rodriguez, {Raymond L.}",

year = "1984",

doi = "10.1016/0378-1119(84)90150-1",

language = "English (US)",

volume = "28",

pages = "331--335",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

AU - Froman, Byron E.

AU - Tait, Robert C.

AU - Kado, Clarence I.

AU - Rodriguez, Raymond L.

PY - 1984

Y1 - 1984

N2 - A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

AB - A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

KW - blunt and cohesive termini

KW - enzyme purification

KW - phage λ

KW - Recombinant DNA

KW - XmaI, SmaI isoschizomers

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U2 - 10.1016/0378-1119(84)90150-1

DO - 10.1016/0378-1119(84)90150-1

M3 - Article

C2 - 6086461

AN - SCOPUS:0021277926

VL - 28

SP - 331

EP - 335

JO - Gene

JF - Gene

SN - 0378-1119

IS - 3

ER -