Abstract
A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 331-335 |
| Number of pages | 5 |
| Journal | Gene |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1984 |
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Keywords
- blunt and cohesive termini
- enzyme purification
- phage λ
- Recombinant DNA
- XmaI, SmaI isoschizomers
ASJC Scopus subject areas
- Genetics
Cite this
Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis. / Froman, Byron E.; Tait, Robert C.; Kado, Clarence I.; Rodriguez, Raymond L.
In: Gene, Vol. 28, No. 3, 1984, p. 331-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis
AU - Froman, Byron E.
AU - Tait, Robert C.
AU - Kado, Clarence I.
AU - Rodriguez, Raymond L.
PY - 1984
Y1 - 1984
N2 - A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.
AB - A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C↓CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.
KW - blunt and cohesive termini
KW - enzyme purification
KW - phage λ
KW - Recombinant DNA
KW - XmaI, SmaI isoschizomers
UR - http://www.scopus.com/inward/record.url?scp=0021277926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021277926&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(84)90150-1
DO - 10.1016/0378-1119(84)90150-1
M3 - Article
C2 - 6086461
AN - SCOPUS:0021277926
VL - 28
SP - 331
EP - 335
JO - Gene
JF - Gene
SN - 0378-1119
IS - 3
ER -