Purification and immune phenotyping of B-1 cells from body cavities of mice

Vanessa Yenson, Nicole Baumgarth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5+ and CD5 B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5 B-1 cells by flow cytometry.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages27-45
Number of pages19
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2270
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • B-1 cell isolation
  • B-cell isolation
  • B-cell subsets
  • Innate-like B cells
  • Natural antibodies
  • Peritoneal cavity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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