Abstract
The ESb-MP cell line is the subclone of a highly malignant variant of murine methylcholanthrene-induced T lymphoma, ESb. When injected in vivo, ESb-MP cells metastasize to the kidney with high frequency, whereas a non- adherent variant, ESb cells, rarely form metastatic foci in the kidney. Our previous results showed that ESb-MP, but not ESb, cells were able to migrate in response to murine kidney-conditioned media (KCM). In an effort to characterize the tumor cell chemoattractant(s) produced by kidney cells, we found that the murine kidney mesangial cell line MES-13 released more chemotactic activity for ESb-MP cells than present in KCM. A major heparin- binding chemotactic activity was purified to homogeneity by sequential fast- performance liquid chromatography and reversed phase high-performance liquid chromatography. Amino acid sequencing of the formic acid-digested active fractions revealed that the purified protein was identical to murine MCP- I(JE) and its activity was neutralized by an anti-MCP-I(JE) antibody. Another chemokine, RANTES, was also purified from MES-13 cell supernatant. The chemotactic activity contained in the MES-13 cell supernatant and in murine KCM was neutralized in part by a combination of anti-MCP-I(JE) and anti- RANTES antibodies. We further examined the differences in the ESb-MP and ESb cells. Binding studies using a variety of radio-iodinated chemokines showed that although both ESb-MP and ESb cells expressed substantial levels of high- affinity binding sites for CC chemokines, only ESb-MP cells migrated in response to CC chemokines and these cells constitutively expressed higher levels of β2 integrin adhesion protein CDIIb than their parental ESb cells. CC chemokines also activated NFκB in ESb-MP but not in ESb cells. Our results indicate that CC chemokines selectively chemoattract and activate ESb-MP cells. Thus, locally produced chemokines, MCP-I(JE) and RANTES in particular, may contribute to the preferential metastasis of ESb-MP cells to the kidneys.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 900-907 |
| Number of pages | 8 |
| Journal | International Journal of Cancer |
| Volume | 75 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Purification and identification of chemokines potentially involved in kidney-specific metastasis by a murine lymphoma variant : Induction of migration and NFκB activation. / Wang, Ji Ming; Chertov, Oleg; Proost, Paul; Li, Jian-Jian; Menton, Patricia; Xu, Luoling; Sozzani, Silvano; Mantovani, Alberto; Gong, Wanghua; Schirrmacher, Volker; Van Damme, Jo; Oppenheim, Joost J.
In: International Journal of Cancer, Vol. 75, No. 6, 1998, p. 900-907.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification and identification of chemokines potentially involved in kidney-specific metastasis by a murine lymphoma variant
T2 - Induction of migration and NFκB activation
AU - Wang, Ji Ming
AU - Chertov, Oleg
AU - Proost, Paul
AU - Li, Jian-Jian
AU - Menton, Patricia
AU - Xu, Luoling
AU - Sozzani, Silvano
AU - Mantovani, Alberto
AU - Gong, Wanghua
AU - Schirrmacher, Volker
AU - Van Damme, Jo
AU - Oppenheim, Joost J.
PY - 1998
Y1 - 1998
N2 - The ESb-MP cell line is the subclone of a highly malignant variant of murine methylcholanthrene-induced T lymphoma, ESb. When injected in vivo, ESb-MP cells metastasize to the kidney with high frequency, whereas a non- adherent variant, ESb cells, rarely form metastatic foci in the kidney. Our previous results showed that ESb-MP, but not ESb, cells were able to migrate in response to murine kidney-conditioned media (KCM). In an effort to characterize the tumor cell chemoattractant(s) produced by kidney cells, we found that the murine kidney mesangial cell line MES-13 released more chemotactic activity for ESb-MP cells than present in KCM. A major heparin- binding chemotactic activity was purified to homogeneity by sequential fast- performance liquid chromatography and reversed phase high-performance liquid chromatography. Amino acid sequencing of the formic acid-digested active fractions revealed that the purified protein was identical to murine MCP- I(JE) and its activity was neutralized by an anti-MCP-I(JE) antibody. Another chemokine, RANTES, was also purified from MES-13 cell supernatant. The chemotactic activity contained in the MES-13 cell supernatant and in murine KCM was neutralized in part by a combination of anti-MCP-I(JE) and anti- RANTES antibodies. We further examined the differences in the ESb-MP and ESb cells. Binding studies using a variety of radio-iodinated chemokines showed that although both ESb-MP and ESb cells expressed substantial levels of high- affinity binding sites for CC chemokines, only ESb-MP cells migrated in response to CC chemokines and these cells constitutively expressed higher levels of β2 integrin adhesion protein CDIIb than their parental ESb cells. CC chemokines also activated NFκB in ESb-MP but not in ESb cells. Our results indicate that CC chemokines selectively chemoattract and activate ESb-MP cells. Thus, locally produced chemokines, MCP-I(JE) and RANTES in particular, may contribute to the preferential metastasis of ESb-MP cells to the kidneys.
AB - The ESb-MP cell line is the subclone of a highly malignant variant of murine methylcholanthrene-induced T lymphoma, ESb. When injected in vivo, ESb-MP cells metastasize to the kidney with high frequency, whereas a non- adherent variant, ESb cells, rarely form metastatic foci in the kidney. Our previous results showed that ESb-MP, but not ESb, cells were able to migrate in response to murine kidney-conditioned media (KCM). In an effort to characterize the tumor cell chemoattractant(s) produced by kidney cells, we found that the murine kidney mesangial cell line MES-13 released more chemotactic activity for ESb-MP cells than present in KCM. A major heparin- binding chemotactic activity was purified to homogeneity by sequential fast- performance liquid chromatography and reversed phase high-performance liquid chromatography. Amino acid sequencing of the formic acid-digested active fractions revealed that the purified protein was identical to murine MCP- I(JE) and its activity was neutralized by an anti-MCP-I(JE) antibody. Another chemokine, RANTES, was also purified from MES-13 cell supernatant. The chemotactic activity contained in the MES-13 cell supernatant and in murine KCM was neutralized in part by a combination of anti-MCP-I(JE) and anti- RANTES antibodies. We further examined the differences in the ESb-MP and ESb cells. Binding studies using a variety of radio-iodinated chemokines showed that although both ESb-MP and ESb cells expressed substantial levels of high- affinity binding sites for CC chemokines, only ESb-MP cells migrated in response to CC chemokines and these cells constitutively expressed higher levels of β2 integrin adhesion protein CDIIb than their parental ESb cells. CC chemokines also activated NFκB in ESb-MP but not in ESb cells. Our results indicate that CC chemokines selectively chemoattract and activate ESb-MP cells. Thus, locally produced chemokines, MCP-I(JE) and RANTES in particular, may contribute to the preferential metastasis of ESb-MP cells to the kidneys.
UR - http://www.scopus.com/inward/record.url?scp=2642704193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2642704193&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0215(19980316)75:6<900::AID-IJC13>3.0.CO;2-6
DO - 10.1002/(SICI)1097-0215(19980316)75:6<900::AID-IJC13>3.0.CO;2-6
M3 - Article
C2 - 9506536
AN - SCOPUS:2642704193
VL - 75
SP - 900
EP - 907
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 6
ER -