Purification and characterization of L-methionine γ-lyase from Brevibacterium linens BL2

Benjamin Dias, Bart C Weimer

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

L-Methionine γ-lyase (EC 4.4.1.11) was purified to homogeneity from Brevibacterium linens BL2, a coryneform bacterium which has been used successfully as an adjunct bacterium to improve the flavor of Cheddar cheese. The enzyme catalyzes the α,γ elimination of methionine to produce methanethiol, α-ketobutyrate, and ammonia. It is a pyridoxal phosphate- dependent enzyme, with a native molecular mass of approximately 170 kDa, consisting of four identical subunits of 43 kDa each. The purified enzyme had optimum activity at pH 7.5 and was stable at pHs ranging from 6.0 to 8.0 for 24 h. The pure enzyme had its highest activity at 25°C but was active between 5 and 50°C. Activity was inhibited by carbonyl reagents, completely inactivated by DL-propargylglycine, and unaffected by metal-chelating agents. The pure enzyme had catalytic properties similar to those of L-methionine γ- lyase from Pseudomonas putida. Its K(m) for the catalysis of methionine was 6.12 mM, and its maximum rate of catalysis was 7.0 μmol min-1 mg-1. The enzyme was active under salt and pH conditions found in ripening Cheddar cheese but susceptible to degradation by intracellular proteases.

Original languageEnglish (US)
Pages (from-to)3327-3331
Number of pages5
JournalApplied and Environmental Microbiology
Volume64
Issue number9
StatePublished - 1998
Externally publishedYes

Fingerprint

Brevibacterium linens
Brevibacterium
Lyases
lyases
Methionine
purification
methionine
enzyme
Enzymes
enzymes
Cheddar cheese
Cheese
catalysis
Catalysis
catalytic activity
Bacteria
pyridoxal phosphate
Pseudomonas putida
bacterium
Pyridoxal Phosphate

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Purification and characterization of L-methionine γ-lyase from Brevibacterium linens BL2. / Dias, Benjamin; Weimer, Bart C.

In: Applied and Environmental Microbiology, Vol. 64, No. 9, 1998, p. 3327-3331.

Research output: Contribution to journalArticle

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