Purification and characterization of granulocytic progenitor cells (CFU-C) from human peripheral blood using immunologic surface markers

Carol M Richman, L. Chess, R. A. Yankee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The concentration of committed granulocytic progenitor cells (CFU-C) in functionally unique subpopulations of human peripheral blood mononuclear cells has been determined by the in vitro methylcellulose assay. Using immunoabsorbent column chromatography and rosette-depletion techniques, we have demonstrated that CFU-C, although not present in either purified T or B lymphocyte populations, are highly concentrated in the 'null' cell population, which lacks sheep erythrocyte receptors and surface immunoglobulin. Further fractionation of this null subset has demonstrated that CFU-C do not bear complement receptors, but require the presence of peripheral blood mononuclear cell feeder layers for maximum proliferation.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalBlood
Volume51
Issue number1
StatePublished - 1978
Externally publishedYes

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Surface Antigens
Purification
Blood Cells
Blood
Stem Cells
Feeder Cells
Complement Receptors
Null Lymphocytes
B-Cell Antigen Receptors
Column chromatography
Methylcellulose
Lymphocytes
Fractionation
Population
Chromatography
Assays
Sheep
B-Lymphocytes
Erythrocytes
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Purification and characterization of granulocytic progenitor cells (CFU-C) from human peripheral blood using immunologic surface markers. / Richman, Carol M; Chess, L.; Yankee, R. A.

In: Blood, Vol. 51, No. 1, 1978, p. 1-8.

Research output: Contribution to journalArticle

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