Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger

Christophe Morisseau, Alain Archelas, Carole Guitton, Didier Faucher, Roland Furstoss, Jacques C. Baratti

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The epoxide hydrolase from Aspergillus niger was purified to homogeneity using a four-step procedure and p-nitrostyrene oxide (pNSO) as substrate. The enzyme was purified 246-fold with 4% activity yield. The protein is a tetramer composed of four identical subunits of molecular mass 45 kDa. Maximum activity was observed at 40 °C, pH 7.0, and with dimethylformamide as cosolvent to dissolve pNSO. Hydrolysis of pNSO was highly enantioselective, with an E value (i.e. enantiomeric ratio) of 40 and a high regioselectivity (97%) for the less hindered carbon atom of the epoxide. This enzyme may be a good biocatalyst for the preparation of enantiopure epoxides or diols.

Original languageEnglish (US)
Pages (from-to)386-395
Number of pages10
JournalEuropean Journal of Biochemistry
Volume263
Issue number2
DOIs
StatePublished - Jul 15 1999

Fingerprint

Epoxide Hydrolases
Aspergillus niger
Aspergillus
Oxides
Purification
Epoxy Compounds
Enzymes
Regioselectivity
Dimethylformamide
Molecular mass
Hydrolysis
Carbon
Atoms
Substrates
Proteins

Keywords

  • Aspergillus niger
  • Diols
  • Enantioselectivity
  • Epoxide hydrolase
  • Regioselectivity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger. / Morisseau, Christophe; Archelas, Alain; Guitton, Carole; Faucher, Didier; Furstoss, Roland; Baratti, Jacques C.

In: European Journal of Biochemistry, Vol. 263, No. 2, 15.07.1999, p. 386-395.

Research output: Contribution to journalArticle

Morisseau, Christophe ; Archelas, Alain ; Guitton, Carole ; Faucher, Didier ; Furstoss, Roland ; Baratti, Jacques C. / Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger. In: European Journal of Biochemistry. 1999 ; Vol. 263, No. 2. pp. 386-395.
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