Purification and architecture of the ubiquitous Wave complex

Alexis Gautreau, Hsin-Yi Henry Ho, Jiaxu Li, Hanno Steen, Steven P. Gygi, Marc W. Kirschner

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

The Wave proteins are major activators of the Arp2/3 complex. The ubiquitous Wave-2 is required for actin polymerization at the leading edge of migrating cells. Here we purify Wave-2 from HeLa cells. Five proteins, Sra, Nap, Wave-2, Abi, and Hspc, are copurified, indicating that they form a tight complex. These proteins are only present in the complexed form, with the exception of Hspc, which displays a free pool. We reconstitute the Wave-2 complex by cotranslating in vitro the five subunits and use this system together with specific immunoprecipitations to study the molecular architecture of the complex. The complex is organized around a core of Nap and Abi. Sra is a peripheral subunit recruited on the Nap side, whereas the Wave and Hspc subunits are recruited on the Abi side of the core.

Original languageEnglish (US)
Pages (from-to)4379-4383
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number13
DOIs
StatePublished - Mar 30 2004
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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