Abstract
Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and inhibitory mechanism of pure curcumin on WT1 isoform-transfected U937 cells. WT1 transfected U937 cells were initially treated for 24 h, with 10 μM pure curcumin. Pure curcumin exhibited a strong inhibitory effect on WT1 (+/+) mRNA level detected by real time PCR. Treatment of WT1 transfected U937 cells with non-cytotoxic doses (10, 15, and 17 μM) of pure curcumin decreased WT1 protein levels in a dose dependent manner. Pure curcumin at the concentration of 15 μM significantly decreased the protein levels of the WT (+/+) isoform in a time-dependent manner. It also decreased exogenous WT1 (+/+) protein half-life. WT1 protein expression was inhibited by protein kinase C inhibitor (GF109203x) suggesting that pure curcumin decreased exogenous WT1 (+/+) expression in transfected U937 cells via protein kinase C during post-translational processing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1846-1856 |
| Number of pages | 11 |
| Journal | African Journal of Pharmacy and Pharmacology |
| Volume | 5 |
| Issue number | 16 |
| DOIs | |
| State | Published - Oct 29 2011 |
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Keywords
- Isoform
- Protein kinase C
- Pure curcumin
- Transfected U937 cell line
- Wilms' tumor (WT1)
ASJC Scopus subject areas
- Pharmaceutical Science
- Pharmacology
Cite this
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells. / Semsri, Suwanna; Anuchapreeda, Songyot; Intasai, Nujeera; Jomgeow, Tanyarat; Tima, Singkome; Sweeney, Colleen A; Limtrakul, Pornngarm.
In: African Journal of Pharmacy and Pharmacology, Vol. 5, No. 16, 29.10.2011, p. 1846-1856.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells
AU - Semsri, Suwanna
AU - Anuchapreeda, Songyot
AU - Intasai, Nujeera
AU - Jomgeow, Tanyarat
AU - Tima, Singkome
AU - Sweeney, Colleen A
AU - Limtrakul, Pornngarm
PY - 2011/10/29
Y1 - 2011/10/29
N2 - Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and inhibitory mechanism of pure curcumin on WT1 isoform-transfected U937 cells. WT1 transfected U937 cells were initially treated for 24 h, with 10 μM pure curcumin. Pure curcumin exhibited a strong inhibitory effect on WT1 (+/+) mRNA level detected by real time PCR. Treatment of WT1 transfected U937 cells with non-cytotoxic doses (10, 15, and 17 μM) of pure curcumin decreased WT1 protein levels in a dose dependent manner. Pure curcumin at the concentration of 15 μM significantly decreased the protein levels of the WT (+/+) isoform in a time-dependent manner. It also decreased exogenous WT1 (+/+) protein half-life. WT1 protein expression was inhibited by protein kinase C inhibitor (GF109203x) suggesting that pure curcumin decreased exogenous WT1 (+/+) expression in transfected U937 cells via protein kinase C during post-translational processing.
AB - Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and inhibitory mechanism of pure curcumin on WT1 isoform-transfected U937 cells. WT1 transfected U937 cells were initially treated for 24 h, with 10 μM pure curcumin. Pure curcumin exhibited a strong inhibitory effect on WT1 (+/+) mRNA level detected by real time PCR. Treatment of WT1 transfected U937 cells with non-cytotoxic doses (10, 15, and 17 μM) of pure curcumin decreased WT1 protein levels in a dose dependent manner. Pure curcumin at the concentration of 15 μM significantly decreased the protein levels of the WT (+/+) isoform in a time-dependent manner. It also decreased exogenous WT1 (+/+) protein half-life. WT1 protein expression was inhibited by protein kinase C inhibitor (GF109203x) suggesting that pure curcumin decreased exogenous WT1 (+/+) expression in transfected U937 cells via protein kinase C during post-translational processing.
KW - Isoform
KW - Protein kinase C
KW - Pure curcumin
KW - Transfected U937 cell line
KW - Wilms' tumor (WT1)
UR - http://www.scopus.com/inward/record.url?scp=80655146486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80655146486&partnerID=8YFLogxK
U2 - 10.5897/AJPP11.149
DO - 10.5897/AJPP11.149
M3 - Article
AN - SCOPUS:80655146486
VL - 5
SP - 1846
EP - 1856
JO - African Journal of Pharmacy and Pharmacology
JF - African Journal of Pharmacy and Pharmacology
SN - 1996-0816
IS - 16
ER -