Pulsed-interleaved excitation FRET measurements on single duplex DNA molecules inside C-shaped nanoapertures

Samantha Fore, Yin Yuen, Lambertus Hesselink, Thomas R Huser

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Single-molecule fluorescence resonant energy transfer (FRET) is a widely accepted method for determining the spatial separation between molecules. In combination with pulsed interleaved excitation (PIE), additional information about the stoichiometry of molecular interactions is obtained. PIE-FRET, however, as implemented with standard confocal optics, requires the dilution of the sample to biologically low concentrations. Here, we show that PIE-FRET measurements inside nanometer-sized apertures yield meaningful biochemical data at 1000× higher concentrations.

Original languageEnglish (US)
Pages (from-to)1749-1756
Number of pages8
JournalNano Letters
Volume7
Issue number6
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

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