Pulse sampling technique

Diego R. Yankelevich, Daniel S. Elson, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


The acquisition of time-resolved data using the pulse sampling technique is perhaps the most intuitive of the data acquisition methods described in this book. A schematic behind the pulse sample principle is shown in Figure 4.1. The sample is excited by a short pulse of light, and the emitted fluorescence is detected by a photodetector and electronics that have a response that is fast enough to record the temporal decay of the fluorescence directly. Typically, these instruments have a response time of 100 ps to a few nanoseconds, and therefore deconvolution, of the instrument response function is often required to extract the intrinsic fluorescence decay. The simplicity of the technology and acquisition method make these instruments well suited to clinical use, and there have been numerous reports of applications in vivo that will be reviewed in this chapter.

Original languageEnglish (US)
Title of host publicationFluorescence Lifetime Spectroscopy and Imaging
Subtitle of host publicationPrinciples and Applications in Biomedical Diagnostics
PublisherCRC Press
Number of pages14
ISBN (Electronic)9781439861684
ISBN (Print)9781439861677
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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