Pulse sampling technique

Diego R. Yankelevich, Daniel S. Elson, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The acquisition of time-resolved data using the pulse sampling technique is perhaps the most intuitive of the data acquisition methods described in this book. A schematic behind the pulse sample principle is shown in Figure 4.1. The sample is excited by a short pulse of light, and the emitted fluorescence is detected by a photodetector and electronics that have a response that is fast enough to record the temporal decay of the fluorescence directly. Typically, these instruments have a response time of 100 ps to a few nanoseconds, and therefore deconvolution, of the instrument response function is often required to extract the intrinsic fluorescence decay. The simplicity of the technology and acquisition method make these instruments well suited to clinical use, and there have been numerous reports of applications in vivo that will be reviewed in this chapter.

Original languageEnglish (US)
Title of host publicationFluorescence Lifetime Spectroscopy and Imaging
Subtitle of host publicationPrinciples and Applications in Biomedical Diagnostics
PublisherCRC Press
Pages87-100
Number of pages14
ISBN (Electronic)9781439861684
ISBN (Print)9781439861677
DOIs
StatePublished - Jan 1 2014

Fingerprint

Fluorescence
sampling
Sampling
fluorescence
acquisition
pulses
circuit diagrams
Schematic diagrams
decay
Deconvolution
Photodetectors
Reaction Time
data acquisition
photometers
Data acquisition
Electronic equipment
Technology
Light
electronics

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Yankelevich, D. R., Elson, D. S., & Marcu, L. (2014). Pulse sampling technique. In Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics (pp. 87-100). CRC Press. https://doi.org/10.1201/b17018

Pulse sampling technique. / Yankelevich, Diego R.; Elson, Daniel S.; Marcu, Laura.

Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. CRC Press, 2014. p. 87-100.

Research output: Chapter in Book/Report/Conference proceedingChapter

Yankelevich, DR, Elson, DS & Marcu, L 2014, Pulse sampling technique. in Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. CRC Press, pp. 87-100. https://doi.org/10.1201/b17018
Yankelevich DR, Elson DS, Marcu L. Pulse sampling technique. In Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. CRC Press. 2014. p. 87-100 https://doi.org/10.1201/b17018
Yankelevich, Diego R. ; Elson, Daniel S. ; Marcu, Laura. / Pulse sampling technique. Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. CRC Press, 2014. pp. 87-100
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