Abstract
Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 28312-28322 |
| Number of pages | 11 |
| Journal | Optics Express |
| Volume | 26 |
| Issue number | 22 |
| DOIs | |
| State | Published - Oct 29 2018 |
| Externally published | Yes |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
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Pilger, C., Hachmeister, H., Greife, P., Weiß, A., Wiebusch, G., & Huser, T. R. (2018). Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express, 26(22), 28312-28322. https://doi.org/10.1364/OE.26.028312