Abstract
Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 28312-28322 |
| Number of pages | 11 |
| Journal | Optics Express |
| Volume | 26 |
| Issue number | 22 |
| DOIs | |
| State | Published - Oct 29 2018 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. / Pilger, Christian; Hachmeister, Henning; Greife, Paul; Weiß, Alex; Wiebusch, Gerd; Huser, Thomas R.
In: Optics Express, Vol. 26, No. 22, 29.10.2018, p. 28312-28322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy
AU - Pilger, Christian
AU - Hachmeister, Henning
AU - Greife, Paul
AU - Weiß, Alex
AU - Wiebusch, Gerd
AU - Huser, Thomas R
PY - 2018/10/29
Y1 - 2018/10/29
N2 - Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
AB - Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=85055858927&partnerID=8YFLogxK
U2 - 10.1364/OE.26.028312
DO - 10.1364/OE.26.028312
M3 - Article
VL - 26
SP - 28312
EP - 28322
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 22
ER -