Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues

Matthew Behrend, Andras Kuthi, Xianyue Gu, P. Thomas Vernier, Laura Marcu, Cheryl M. Craft, Martin A. Gundersen

Research output: Contribution to journal › Article

66 Citations (Scopus)

Abstract

This paper describes three pulse generators: a spark gap switched coaxial cable, a spark gap switched Blumlein, and a solid state modulator, developed for applying ultrashort electrical pulses to biological materials in culture. Recent research has shown that ultrashort pulsed electric fields can induce apoptosis in biological cells, and that pulses as short as 10 ns with field amplitude greater than 1 MV/m cause membrane phospholipid rearrangement and activation of the effector enzymes of apoptosis. Pulses of very short duration use only tens of mJ per mL per pulse to induce apoptosis and other intracellular effects without causing thermal trauma. The pulse generators discussed here, each of a different topology, deliver ns pulsed electric fields (nsPEF) to cells in liquid suspension, and can be modified to drive electrodes for external, surgical, or endoscopie treatment of tissues in situ.

Original languageEnglish (US)
Pages (from-to)820-825
Number of pages6
JournalIEEE Transactions on Dielectrics and Electrical Insulation
Volume10
Issue number5
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Pulse generators
Cell death
Electric fields
Tissue
Electric sparks
Coaxial cables
Phospholipids
Biological materials
Modulators
Enzymes
Chemical activation
Topology
Membranes
Electrodes
Liquids

Keywords

  • Apoptosis
  • Blumlein
  • Electric field
  • Electroperturbation
  • Fluorescence microscopy
  • Nanosecond
  • NsPEF
  • Phospholipid inversion
  • Pulse forming network
  • Pulse generator
  • Pulsed electric field
  • Real-time
  • Ultrashort

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Behrend, M., Kuthi, A., Gu, X., Vernier, P. T., Marcu, L., Craft, C. M., & Gundersen, M. A. (2003). Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues. IEEE Transactions on Dielectrics and Electrical Insulation, 10(5), 820-825. https://doi.org/10.1109/TDEI.2003.1237331

Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues. / Behrend, Matthew; Kuthi, Andras; Gu, Xianyue; Vernier, P. Thomas; Marcu, Laura; Craft, Cheryl M.; Gundersen, Martin A.

In: IEEE Transactions on Dielectrics and Electrical Insulation, Vol. 10, No. 5, 10.2003, p. 820-825.

Research output: Contribution to journal › Article

Behrend, M, Kuthi, A, Gu, X, Vernier, PT, Marcu, L, Craft, CM & Gundersen, MA 2003, 'Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues', IEEE Transactions on Dielectrics and Electrical Insulation, vol. 10, no. 5, pp. 820-825. https://doi.org/10.1109/TDEI.2003.1237331
Behrend M, Kuthi A, Gu X, Vernier PT, Marcu L, Craft CM et al. Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues. IEEE Transactions on Dielectrics and Electrical Insulation. 2003 Oct;10(5):820-825. https://doi.org/10.1109/TDEI.2003.1237331
Behrend, Matthew ; Kuthi, Andras ; Gu, Xianyue ; Vernier, P. Thomas ; Marcu, Laura ; Craft, Cheryl M. ; Gundersen, Martin A. / Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues. In: IEEE Transactions on Dielectrics and Electrical Insulation. 2003 ; Vol. 10, No. 5. pp. 820-825.
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