Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues

Matthew Behrend; Andras Kuthi; Xianyue Gu; P. Thomas Vernier; Laura Marcu; Cheryl M. Craft; Martin A. Gundersen

doi:10.1109/TDEI.2003.1237331

Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues

Matthew Behrend, Andras Kuthi, Xianyue Gu, P. Thomas Vernier, Laura Marcu, Cheryl M. Craft, Martin A. Gundersen

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

This paper describes three pulse generators: a spark gap switched coaxial cable, a spark gap switched Blumlein, and a solid state modulator, developed for applying ultrashort electrical pulses to biological materials in culture. Recent research has shown that ultrashort pulsed electric fields can induce apoptosis in biological cells, and that pulses as short as 10 ns with field amplitude greater than 1 MV/m cause membrane phospholipid rearrangement and activation of the effector enzymes of apoptosis. Pulses of very short duration use only tens of mJ per mL per pulse to induce apoptosis and other intracellular effects without causing thermal trauma. The pulse generators discussed here, each of a different topology, deliver ns pulsed electric fields (nsPEF) to cells in liquid suspension, and can be modified to drive electrodes for external, surgical, or endoscopie treatment of tissues in situ.

Original language	English (US)
Pages (from-to)	820-825
Number of pages	6
Journal	IEEE Transactions on Dielectrics and Electrical Insulation
Volume	10
Issue number	5
DOIs	https://doi.org/10.1109/TDEI.2003.1237331
State	Published - Oct 2003
Externally published	Yes

Keywords

Apoptosis
Blumlein
Electric field
Electroperturbation
Fluorescence microscopy
Nanosecond
NsPEF
Phospholipid inversion
Pulse forming network
Pulse generator
Pulsed electric field
Real-time
Ultrashort

ASJC Scopus subject areas

Electrical and Electronic Engineering

Access to Document

10.1109/TDEI.2003.1237331

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Pulse Generators for Pulsed Electric Field Exposure of Biological Cells and Tissues. / Behrend, Matthew; Kuthi, Andras; Gu, Xianyue; Vernier, P. Thomas; Marcu, Laura; Craft, Cheryl M.; Gundersen, Martin A.

In: IEEE Transactions on Dielectrics and Electrical Insulation, Vol. 10, No. 5, 10.2003, p. 820-825.

Research output: Contribution to journal › Article › peer-review

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abstract = "This paper describes three pulse generators: a spark gap switched coaxial cable, a spark gap switched Blumlein, and a solid state modulator, developed for applying ultrashort electrical pulses to biological materials in culture. Recent research has shown that ultrashort pulsed electric fields can induce apoptosis in biological cells, and that pulses as short as 10 ns with field amplitude greater than 1 MV/m cause membrane phospholipid rearrangement and activation of the effector enzymes of apoptosis. Pulses of very short duration use only tens of mJ per mL per pulse to induce apoptosis and other intracellular effects without causing thermal trauma. The pulse generators discussed here, each of a different topology, deliver ns pulsed electric fields (nsPEF) to cells in liquid suspension, and can be modified to drive electrodes for external, surgical, or endoscopie treatment of tissues in situ.",

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