Pulsatile Lipid Vesicles under Osmotic Stress

Morgan Chabanon, James C.S. Ho, Bo Liedberg, Atul N. Parikh, Padmini Rangamani

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The response of lipid bilayers to osmotic stress is an important part of cellular function. Recent experimental studies showed that when cell-sized giant unilamellar vesicles (GUVs) are exposed to hypotonic media, they respond to the osmotic assault by undergoing a cyclical sequence of swelling and bursting events, coupled to the membrane's compositional degrees of freedom. Here, we establish a fundamental and quantitative understanding of the essential pulsatile behavior of GUVs under hypotonic conditions by advancing a comprehensive theoretical model of vesicle dynamics. The model quantitatively captures the experimentally measured swell-burst parameters for single-component GUVs, and reveals that thermal fluctuations enable rate-dependent pore nucleation, driving the dynamics of the swell-burst cycles. We further extract constitutional scaling relationships between the pulsatile dynamics and GUV properties over multiple timescales. Our findings provide a fundamental framework that has the potential to guide future investigations on the nonequilibrium dynamics of vesicles under osmotic stress.

Original languageEnglish (US)
Pages (from-to)1682-1691
Number of pages10
JournalBiophysical Journal
Volume112
Issue number8
DOIs
StatePublished - Apr 25 2017

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Unilamellar Liposomes
Osmotic Pressure
Lipids
Lipid Bilayers
Giant Cells
Theoretical Models
Hot Temperature
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Chabanon, M., Ho, J. C. S., Liedberg, B., Parikh, A. N., & Rangamani, P. (2017). Pulsatile Lipid Vesicles under Osmotic Stress. Biophysical Journal, 112(8), 1682-1691. https://doi.org/10.1016/j.bpj.2017.03.018

Pulsatile Lipid Vesicles under Osmotic Stress. / Chabanon, Morgan; Ho, James C.S.; Liedberg, Bo; Parikh, Atul N.; Rangamani, Padmini.

In: Biophysical Journal, Vol. 112, No. 8, 25.04.2017, p. 1682-1691.

Research output: Contribution to journalArticle

Chabanon, M, Ho, JCS, Liedberg, B, Parikh, AN & Rangamani, P 2017, 'Pulsatile Lipid Vesicles under Osmotic Stress', Biophysical Journal, vol. 112, no. 8, pp. 1682-1691. https://doi.org/10.1016/j.bpj.2017.03.018
Chabanon M, Ho JCS, Liedberg B, Parikh AN, Rangamani P. Pulsatile Lipid Vesicles under Osmotic Stress. Biophysical Journal. 2017 Apr 25;112(8):1682-1691. https://doi.org/10.1016/j.bpj.2017.03.018
Chabanon, Morgan ; Ho, James C.S. ; Liedberg, Bo ; Parikh, Atul N. ; Rangamani, Padmini. / Pulsatile Lipid Vesicles under Osmotic Stress. In: Biophysical Journal. 2017 ; Vol. 112, No. 8. pp. 1682-1691.
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